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Example sentences for: linkers
How can you use “linkers” in a sentence? Here are some example sentences to help you improve your vocabulary:
These modifications include the direct binding of mRNA to magnetic oligo(dT) beads before the reverse transcription step, and a great reduction in the quantity of linkers used to generate ditags.
This is analogous to the use of the bovine pancreatic trypsin inhibitor for internal insertions [ 21 ] . The transposons described here could potentially be improved upon by optimizing the length and flexibility of the linkers between the target and the GFP.
Two synthetic transposons have been reported that can produce GFP fusion proteins [ 9 10 ] . The design of these transposons included additional protein domains or linkers between the GFP and the target protein, however, and little is known about how many of the resulting proteins continued to function.
Sodium salts of each compound were titrated into assays of purified Fhit [ 16 ] with 1.8 μM fluorescent substrate, Appp-S-(4-4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl)methylaminoacetyl (ApppBODIPY) [ 10 ] , and competitive K i values were obtained by calculating the inhibitor concentration-dependence in reduction of k cat / K m (apparent) for substrate hydrolysis [ 10 ] . As shown in Table 1, inhibitors 2b and 3b with two-carbon diol linkers were either only as inhibitory as the natural substrate 1 or substantially less so.
RNA was reverse transcribed into cDNA using random hexamer primers in the presence of 5-aminoallyl-dUTP; Cy3 and Cy5 dyes were covalently coupled to the incorporated aminoallyl linkers in a subsequent labeling reaction.
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