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Example sentences for: linkers
How can you use “linkers” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first protocol uses a ligation-mediated PCR strategy (LM-PCR) in which ChIP DNA is ligated to short DNA linkers, and then PCR amplified using the linker sequence for priming [ 4 ] . The second uses a random amplification strategy (R-PCR) in which template DNA is first copied using a specialized primer with degenerate 3' sequence and known 5' sequence, and then PCR amplified using the known 5' sequence for priming [ 5 8 9 ] . In both cases, the PCR steps yield quantities of nucleic acid sufficient for microarray analysis.
This is analogous to the use of the bovine pancreatic trypsin inhibitor for internal insertions [ 21 ] . The transposons described here could potentially be improved upon by optimizing the length and flexibility of the linkers between the target and the GFP.
These modifications include the direct binding of mRNA to magnetic oligo(dT) beads before the reverse transcription step, and a great reduction in the quantity of linkers used to generate ditags.
We reasoned that a Tn 5 transposon [ 11 12 ] could be designed that would generate GFP fusion proteins with relatively short linkers (~7 amino acids) between the GFP and the host protein analogous to GFP fusion proteins that have already been shown to function [ 6 8 ] . To test this approach, we targeted the G protein subunit α s and the glutamate receptor subunit GluR1.
Two synthetic transposons have been reported that can produce GFP fusion proteins [ 9 10 ] . The design of these transposons included additional protein domains or linkers between the GFP and the target protein, however, and little is known about how many of the resulting proteins continued to function.
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