Words similar to licl

Example sentences for: licl

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LiCl has previously been shown to mimic activation of Wnt-1 signaling by stabilizing beta-catenin through its inhibition of glycogen synthase kinase-3β (GSK-3β), a kinase that phosphorylates beta-catenin and promotes its degradation via the ubiquitin proteasome pathway [ 39 ] . C57mg cells treated with LiCl exhibited elevated levels of mesothelin but not SL-1 (Figure 2, left panel).
PMA, a phorbol ester, activates PKC and has previously been shown to activate SL-1 [ 40 ] . It appears that mesothelin is activated by Wnt-1, which can be mimicked by LiCl, and that SL-1 is activated by Wnt-5a in a beta-catenin-independent manner, which can be mimicked by activating PKC.
For Li+ and PMA stimulation experiments, C57mg cells were incubated with 10 mM LiCl, 10 mM KCl as a control, or 1 μM PMA for 6 hours.
Isolation of total cellular RNA using a urea/LiCl protocol and gel electrophoresis was performed as described previously [ 65 ] . Gels were transferred onto Amersham Hybond N+ nylon membranes in 20 × SSC and prehybridized in 4 × SSC, 1 × Denhardt's solution, 0.5% SDS for 2 h at 68°C.
The plasmids used for transfection were prepared by the alkaline/PEG/LiCl method.