Words similar to lemaf
Example sentences for: lemaf
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A yeast two-hybrid screen was performed to identify interaction partners of the tomato nuclear envelope-associated protein LeMAF1 [ 36 ] . A prey plasmid (pAD-LeFPP) was identified that led to activation of the two reporter genes HIS and LacZ after retransformation with the pBD-LeMAF1 bait.
The complete cDNA of LeMAF1 (GenBank accession number AF118113) was cloned as an EcoRI/XhoI fragment between the EcoRI and SalI sites of the bait vector pBD-GAL4 (Statagene).
Its N-terminus has 28% amino acid identity with LeMAF1 and 30% with AtMAF1 (Fig.
AtMAF1 is one of three Arabidopsis homologs of LeMAF1 (Patel and Meier, unpublished results), and has 45% amino acid identity with LeMAF1.