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Example sentences for: lbnl

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LBNL and the HGSC used slightly different strategies for gap filling.
At LBNL, 340 BACs (40.
With an estimated gap size, LBNL used one of five gap-filling methods to determine their sequence: first, gaps less than 500 bp were sequenced using custom primers designed by consed and a 3-kb or 10-kb plasmid as DNA template; second, gaps between 500 bp and 2.5 kb that were spanned by a 3-kb clone were completed by sequencing the 3-kb clone using a transposon-based sequencing strategy, described below; third, gaps between 2.5 kb and 10 kb that were spanned by a 10-kb clone were completed by sequencing the 10-kb clone using a transposon-mediated sequencing strategy; fourth, gaps larger than 10 kb were completed by sequentially sequencing multiple 10-kb clones to walk across the gap.
At LBNL, Release 3 BAC sequence assemblies were also compared to the Release 2 sequence from that region using Sim4 to analyze the large-scale assembly, and Sim3 to identify base-pair differences.
The programs Assemble-arms (LBNL) or BACstitcher (HGSC) was used to generate a FASTA file of the chromosome arms, starting with the first BAC in the tiling path and adding the unique sequence from the adjoining BAC to extend the sequence contig.