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Example sentences for: lbnl

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0. At LBNL, BAC end sequences were manually tagged, and if the assembly was in more than one contig, gap sizes were estimated using Consed and phrapview version 0.960731 [ 10].
At LBNL, BAC assemblies were viewed using phrapview, which was customized to recognize our 2-kb, 3-kb and 10-kb subclones and display the paired-end relationships with a color-coded key.
LBNL employed a γδ transposon-based strategy to sequence 3-kb clones essentially as described [ 38].
At LBNL, draft sequence from neighboring BACs was also included in each assembly.
With an estimated gap size, LBNL used one of five gap-filling methods to determine their sequence: first, gaps less than 500 bp were sequenced using custom primers designed by consed and a 3-kb or 10-kb plasmid as DNA template; second, gaps between 500 bp and 2.5 kb that were spanned by a 3-kb clone were completed by sequencing the 3-kb clone using a transposon-based sequencing strategy, described below; third, gaps between 2.5 kb and 10 kb that were spanned by a 10-kb clone were completed by sequencing the 10-kb clone using a transposon-mediated sequencing strategy; fourth, gaps larger than 10 kb were completed by sequentially sequencing multiple 10-kb clones to walk across the gap.