Example sentences for: layered

How can you use “layered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In brief, after three washes with phosphate-buffered saline (PBS), fixed cells in each smear were layered with the primary antibody against P-gp (1:20 dilution), GSTπ (1:25 dilution) and TopoIIα (1:50 dilution) and incubated overnight (a minimum of 18 h).

  • In those seven lines, freighted with the layered voice of European culture, Dante's vision of paradise is invoked both explicitly, in the "concentric radiance" of the last canto of his Paradiso , and implicitly, in his braided terza rima (three-line stanzas, rhyming--loosely here--aba, bcb, etc.).

  • It was gently layered over equal quantities of Ficoll-Hypaque density gradient and centrifuged at 1800 rpm for 30 min.

  • The supernatant was layered on a 30% sucrose cushion and centrifuged at 245,000×g for 2 h at 4°C.

  • Fresh medium was layered above the agarose every 3-5 days.


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