Words similar to large-budded
Example sentences for: large-budded
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When cells harboring conditional-lethal temperature-sensitive (ts) mutations in any of these proteins are shifted to the restrictive temperature, they uniformly arrest in late anaphase as large-budded cells with segregated chromosomes, fully elongated microtubule spindles, and in all tested cases, elevated Cdc28/Clb2 protein kinase activity (Cdc28 is the major cyclin-dependent kinase Cdk1 and Clb2 the major mitosis-specific cyclin of budding yeast) (reviewed in [ 1 ] , [ 2 ] ).
In constrast, the SH3-domain mutation had no obvious effect on the localization of Boi1-GFP to the bud in either small- or large-budded cells (Figs.
, analyzing first only unbudded cells, then only small-budded cells, and then only large-budded cells).
However, this mutation increased the frequency of localization to the periphery of large-budded cells, and it did so to a degree that approximated that to which it caused a decrease in the frequency of localization to the neck: in large-budded cells, whereas wild-type Boi1-GFP was localized to the periphery of the mother part of the cell in only 3% of the cells and to the neck in 31% of the cells, Boi1-S-GFP was localized to the periphery of the mother in 22% of the cells (and was never localized to the neck).
A more striking effect of the KK, TK, and KKTK substitutions on the localization of Boi1-GFP was seen in cells that contained large buds: Boi1-TK-GFP was concentrated in the bud in only 4% of large-budded cells, and Boi1-KK-GFP and Boi1-KKTK-GFP were concentrated in the bud in less than 1% of such cells (Fig.
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