Words similar to lambda
Example sentences for: lambda
How can you use “lambda” in a sentence? Here are some example sentences to help you improve your vocabulary:
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.
The search for additional subunit A genes was conducted using whole genome blot analysis, screening a lambda PRL-2 cDNA library, intron length polymorphism analysis, and extensive computer searches of the Arabidopsis Expressed Sequence Tag database, GenBank, and the Arabidopsis Genome Initiative.
Mouse Npm3 is located approximately 5 kb upstream of the Fgf8 gene in the same transcriptional orientation [ 20 21 ] . To determine if human NPM3 is similarly located and to identify genomic clones of NPM3, we analyzed FGF8-containing human genomic lambda clones [ 22 ] by Southern blotting using a mouse Npm3 cDNA probe.
Positively hybridizing plaques were purified by limiting dilution and phage DNA was isolated and purified with the Lambda Maxi Kit (Qiagen).
Treatment of agarose slices linearized the plasmid DNA and allowed for plasmid size determination using a concatomerized bacteriophage lambda standard (New England BioLabs, Beverly, MA) (5).
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