Example sentences for: laemmli

How can you use “laemmli” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Polyacrylamide gel electrophoresis and Western blot analyses were performed as described [ 13 23 ] . Briefly, samples were electrophoresed on 10% resolving sodium dodecyl sulphate-polyacrylamide gels (SDS/PAGE) according to the method of Laemmli [ 35 ] . Proteins were then electrotransferred onto nitrocellulose membranes and the membranes were incubated in TBST buffer containing 5% nonfat dry milk for 1 hour to block nonspecific protein binding.

  • Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.

  • Samples were boiled in 1× Laemmli buffer for 3 minutes, separated by SDS-PAGE, and blotted to polyvinylidene difluoride membranes.

  • After washing, the beads were boiled for 5 min in Laemmli sample buffer.

  • 5 μl of 2x Laemmli buffer with 8 M Urea [ 47] was added per brain or head, respectively.


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