Words similar to laemmli
Example sentences for: laemmli
How can you use “laemmli” in a sentence? Here are some example sentences to help you improve your vocabulary:
After washing, the beads were boiled for 5 min in Laemmli sample buffer.
Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.
10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.
Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.
SDS-PAGE was performed with a Mini-PROTEIN II cell (Bio-Rad) using the method of Laemmli [ 44].
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