Words similar to laemmli

Example sentences for: laemmli

How can you use “laemmli” in a sentence? Here are some example sentences to help you improve your vocabulary:

10 μL of supernatant was removed, mixed with 20 μL of Laemmli sample buffer containing β-mercaptoethanol (19:1) and incubated at room temperature for five minutes.
After preincubation with protein A-sepharose 4CL, the cell extract was incubated with specific mAb directly conjugated to sepharose 4B for 60 min, washed 3 times with the appropriate lysis buffer and eluted with Laemmli sample buffer.
All samples were boiled in Laemmli denaturing buffer and analyzed by Western blotting.
Polyacrylamide gel electrophoresis and Western blot analyses were performed as described [ 13 23 ] . Briefly, samples were electrophoresed on 10% resolving sodium dodecyl sulphate-polyacrylamide gels (SDS/PAGE) according to the method of Laemmli [ 35 ] . Proteins were then electrotransferred onto nitrocellulose membranes and the membranes were incubated in TBST buffer containing 5% nonfat dry milk for 1 hour to block nonspecific protein binding.
Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.