Example sentences for: laemmli

How can you use “laemmli” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing, the beads were boiled for 5 min in Laemmli sample buffer.

  • Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.

  • 10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.

  • Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.

  • SDS-PAGE was performed with a Mini-PROTEIN II cell (Bio-Rad) using the method of Laemmli [ 44].


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