Words similar to laemmli
Example sentences for: laemmli
How can you use “laemmli” in a sentence? Here are some example sentences to help you improve your vocabulary:
For western blot analysis, IP were resuspended in 20 μl 2 × SDS/sample (Laemmli) buffer and the supernatants were resolved by SDS-PAGE.
10 μL of supernatant was removed, mixed with 20 μL of Laemmli sample buffer containing β-mercaptoethanol (19:1) and incubated at room temperature for five minutes.
Samples were boiled in 1× Laemmli buffer for 3 minutes, separated by SDS-PAGE, and blotted to polyvinylidene difluoride membranes.
Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.
Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.