Words similar to laemmli

Example sentences for: laemmli

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Protein extracts (25 μg) were mixed with an equal volume of 2× Laemmli's sample buffer.
Laemmli electrophoresis sample buffer (5×) was added to the lysates, and SDS-polyacrylamide gel electrophoresis and immunoblotting were performed using 35 μg lysate protein, phospho-specific antibodies for ERK and p38, and rabbit polyclonal antibody for cPLA 2 [ 21 ] .
10 μL of supernatant was removed, mixed with 20 μL of Laemmli sample buffer containing β-mercaptoethanol (19:1) and incubated at room temperature for five minutes.
Polyacrylamide gel electrophoresis and Western blot analyses were performed as described [ 13 23 ] . Briefly, samples were electrophoresed on 10% resolving sodium dodecyl sulphate-polyacrylamide gels (SDS/PAGE) according to the method of Laemmli [ 35 ] . Proteins were then electrotransferred onto nitrocellulose membranes and the membranes were incubated in TBST buffer containing 5% nonfat dry milk for 1 hour to block nonspecific protein binding.
After washing, the beads were boiled for 5 min in Laemmli sample buffer.