Example sentences for: laemmli

How can you use “laemmli” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For western blot analysis, IP were resuspended in 20 μl 2 × SDS/sample (Laemmli) buffer and the supernatants were resolved by SDS-PAGE.

  • 10 μL of supernatant was removed, mixed with 20 μL of Laemmli sample buffer containing β-mercaptoethanol (19:1) and incubated at room temperature for five minutes.

  • Samples were boiled in 1× Laemmli buffer for 3 minutes, separated by SDS-PAGE, and blotted to polyvinylidene difluoride membranes.

  • Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.

  • Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.


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