Words similar to laboratory-to-laboratory
Example sentences for: laboratory-to-laboratory
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Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
Some techniques have been useful for the identification and/or differentiation of Brucella species and biovars [ 1 6 7 23 24 ] , but few tools have been available for the epidemiological trace-back of new infections in livestock [ 1 2 3 4 5 ] . Except for biotyping, none of these more discriminating techniques has been universally accepted, probably because of concerns about laboratory-to-laboratory reproducibility.
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