Words similar to l-
Example sentences for: l-
How can you use “l-” in a sentence? Here are some example sentences to help you improve your vocabulary:
For determination of basal gene-expression profiles without androgen stimulation, fibroblasts were cultured on 150-mm plastic dishes at 37°C with 5% CO 2 . To eliminate possible artifacts due to differing states of proliferation, cells were grown to confluence, at which point they enter G 0 arrest [ 31 ] . They were maintained in phenol-red-free DMEM F12 (Dulbecco's modified Eagle Medium with the nutrient mix F12; Gibco) containing L-glutamine, 15 mM Hepes buffer, penicillin/streptomycin (Gibco) and 12.
The cells were lysed in agarose gel by a modification of the method of Kusano et al . [ 35 ] . Cells were grown in 5 ml of L-broth with or without antibiotics to an OD 660 of 0.4 and were harvested.
The FLS cultures were established from synovial tissues obtained during joint replacement surgery in RA patients [ 6 ] . The FLS were cultured in DMEM plus 10% heat-inactivated human AB serum (BioWhittaker, Walkersville, MD, USA), 10% fetal bovine serum, penicillin, streptomycin, and L-glutamine.
For example, RAG1 has been reported to interact with the tandem HMG-boxes of HMGB1 or HMGB2 [ 6 ] . Where it has been studied, HMG-boxes have been shown to be structurally related, forming three α-helices in a characteristic L-shaped structure [ 3 7 ] . The distortion of DNA by the HMG-box is primarily mediated through contacts with the minor groove of the DNA helix, potentially allowing simultaneous binding of other transcriptional regulators to the DNA [ 8 9 ] . The HMG-box family has often been subdivided based on DNA binding properties.
L-glutamate (Fisher Scientific, Pittsburgh, PA) was applied at a concentration of 100 μM in all experiments.
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