Words similar to kxd
Example sentences for: kxd
How can you use “kxd” in a sentence? Here are some example sentences to help you improve your vocabulary:
An extensive body of published work supports the notion that Rab proteins bearing mutations in the N(T)KxD nucleotide binding motif function as dominant-suppressors of their endogenous cellular counterparts.
Previous studies have shown that Rab24 exhibits several unusual characteristics that distinguish it from other members of the Rab GTPase family [ 25 ] . The studies described in this report demonstrate that putative dominant-negative mutant forms of Rab24, bearing single amino acid substitutions in the conserved N(T)KxD nucleotide binding motif ( e.g. , D123I and T120A), are targeted to numerous inclusion bodies that disrupt the nuclear envelope and enter the nuclear compartment.
We also verified that the unique behavior of the Rab24 mutant was not related specifically to the substitution at the aspartate position in the N(T)KxD motif.
Rab24 contains a unique arginine-rich insert, 126EEDRRRRR 133 [ 25 ] in the domain cognate to loop-8 in H-Ras [ 34 ] . The latter sequence is reminiscent of unusual arginine-rich nuclear localization signals that allow proteins like HIV tet and rev to undergo nuclear translocation by association with importin-β, in the absence of importin-α [ 45 46 ] . Since the loop-8 insert in Rab24 lies adjacent to the conserved N(T)KxD nucleotide binding cassette, we hypothesized that conformational changes caused by the D123I substitution might render the Rab24 mutant capable of disrupting the nuclear import machinery [ 47 ] . To test this possibility, we co-expressed mycRab24wt or mycRab24(D123I) with GR-GFP, a fusion between the glucocorticoid receptor and green fluorescent protein.
To test the possibility that protein aggregates might be formed when any Rab GTPase is overexpressed with a mutation that impairs nucleotide binding, we examined the localization of four additional members of the Rab family containing amino acid substitutions in the N(T)KxD motif.
Loading...