Words similar to kxd
Example sentences for: kxd
How can you use “kxd” in a sentence? Here are some example sentences to help you improve your vocabulary:
Rab24 contains a unique arginine-rich insert, 126EEDRRRRR 133 [ 25 ] in the domain cognate to loop-8 in H-Ras [ 34 ] . The latter sequence is reminiscent of unusual arginine-rich nuclear localization signals that allow proteins like HIV tet and rev to undergo nuclear translocation by association with importin-β, in the absence of importin-α [ 45 46 ] . Since the loop-8 insert in Rab24 lies adjacent to the conserved N(T)KxD nucleotide binding cassette, we hypothesized that conformational changes caused by the D123I substitution might render the Rab24 mutant capable of disrupting the nuclear import machinery [ 47 ] . To test this possibility, we co-expressed mycRab24wt or mycRab24(D123I) with GR-GFP, a fusion between the glucocorticoid receptor and green fluorescent protein.
Similar mutations in the N(T)KxD motif of other Rab proteins typically yield soluble cytoplasmic proteins.
To test the possibility that protein aggregates might be formed when any Rab GTPase is overexpressed with a mutation that impairs nucleotide binding, we examined the localization of four additional members of the Rab family containing amino acid substitutions in the N(T)KxD motif.
Therefore, it appears that although the C-terminal domain of Rab24 is required to promote its incorporation into inclusion bodies, it does so only when expressed in the context of a Rab GTPase with a mutation in the N(T)KxD nucleotide binding motif.
An extensive body of published work supports the notion that Rab proteins bearing mutations in the N(T)KxD nucleotide binding motif function as dominant-suppressors of their endogenous cellular counterparts.
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