Words similar to klenow
Example sentences for: klenow
How can you use “klenow” in a sentence? Here are some example sentences to help you improve your vocabulary:
pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
This adapter is annealed to the genomic DNA fragments, and Klenow is added for second strand synthesis.
pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
This was inserted downstream of the SA in pPacSA digested with SalI, Klenow filled, and then cut with KpnI.
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