Example sentences for: klenow

How can you use “klenow” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.

  • The double stranded oligonucleotide probes were end-filled with Klenow DNA polymerase and α- 32P-dGTP.

  • The loxP - neo -tpA- loxP was excised from plasmid PGKneotpAlox2 by cutting with NotI, Klenow filling, and then cutting with KpnI.

  • The construct was produced by cutting pSG5 fs α 1S with AgeI and HindIII enzymes and filling-in the overhangs with klenow fragments.

  • The PCR product was digested with either the Eco RI or Xba I restriction enzyme, end-labeled with [αP 32]-dATP (ICN, Inc.) using the Klenow fragment of DNA polymerase I, and purified using a PCR clean-up kit (Qiagen).


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