Example sentences for: klenow

How can you use “klenow” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following hybridization, the gels were washed 2× in Wash1E and then equilibrated in Klenow extension buffer (50 mM Tris-HCl, pH 7.5, 5 mM MgCl 2 , 0.01% Triton X-100) for 1 minute.

  • A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.

  • Finally, the deleted open reading frame-containing fragment was excised using Sph I and Msc I, and inserted into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.

  • The KIAA1194 cDNA was first 3'-truncated upon restriction with Bsm I (nt 3642) and Sac II (in pBluescript II SK+ vector), Klenow treatment and self-ligation, to remove undesirable Eco NI restriction sites.

  • Unamplified DNA samples for labeling were generated from 1 μg restricted DNA using Klenow fragment and random primers, and incorporating amino-allyl dUTP, as described at http://www.microarrays.org.


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