Words similar to klenow

Example sentences for: klenow

How can you use “klenow” in a sentence? Here are some example sentences to help you improve your vocabulary:

To make the ROSA26 genomic sequence compatible with the pBigT plasmid, pROSA26-1 was digested with XbaI, Klenow filled and a linker (PacI, SwaI, AscI) inserted.
In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.
A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
This was inserted downstream of the SA in pPacSA digested with SalI, Klenow filled, and then cut with KpnI.
To generate pGaL4-hCCR4, a fragment corresponding to the entire hCCR4 coding sequence (with the exception of the initiation codon) was obtained upon digestion of the KIAA1194 cDNA (accession number AB033020, gift from the Kazusa Research Institute, putative start codon -Met 19-positioned by homology with mCCR4) by Sph I and Msc I (nt 423 to 2172 in KIAA1194), and was inserted in-frame into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.