Words similar to kktk

Example sentences for: kktk

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To investigate whether binding to phospholipid may be important for Boi1 function, we used a red/white, colony-sectoring assay to test whether the KK, TK, and KKTK mutant versions of Boi1 could substitute in function for wild-type BOI1 and BOI2 . For this analysis, we used strain PY967, which lacks the genomic copies of BOI1 and BOI2 and which is kept alive by BOI1 -bearing plasmid pPB799.
The KK, TK, and KKTK mutant versions of Boi1-PH contain substitutions at positions in the PH domain that were predicted to be involved in binding PIP 2 . PIP 2 /PS vesicles did not stimulate the proteolysis of any of these mutant versions of Boi1-PH, suggesting that each mutant is indeed impaired in the ability to associate with PIP 2 and/or PS.
We found that, in conditions in which PIP 2 stimulated proteolysis of most of the wild-type Boi1-PH, PIP 2 did not stimulate the proteolysis of the TK, KK, and KKTK versions of Boi1-PH (Fig.
Thus, although the KK, TK, and KKTK mutant proteins all were able to localize to the bud in small-budded cells, they were localized and/or retained there less efficiently than was wild-type Boi1-GFP.
As expected, given that the TK substitutions were sufficient to severely affect pelleting with PS vesicles, none of the Boi1-PH(KKTK) was seen to co-sediment with PS vesicles (Fig.