Words similar to kktk
Example sentences for: kktk
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None of the Boi1-PH(KKTK) was detected in the pellet fraction even when using vesicles that contained PIP 2 (Fig.
The myristoyl group-accepting sequence did not completely restore function to the KK and KKTK versions of Boi1-PH, however, raising the possibility that, rather than simply promoting general association with membrane, one role of binding to PIP 2 may be to regulate some other behavior of Boi1 (e.g.
To investigate whether binding to phospholipid may be important for Boi1 function, we used a red/white, colony-sectoring assay to test whether the KK, TK, and KKTK mutant versions of Boi1 could substitute in function for wild-type BOI1 and BOI2 . For this analysis, we used strain PY967, which lacks the genomic copies of BOI1 and BOI2 and which is kept alive by BOI1 -bearing plasmid pPB799.
The wild-type, KK, TK, and KKTK versions of Boi1-PH
The KK (as well as the KKTK) version of Boi1 appeared to be completely non-functional, pointing to the possibility that binding specifically to PIP 2 (as opposed to binding to acidic phospholipids generally) may be critical for Boi1 function.
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