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Example sentences for: kktk

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None of the Boi1-PH(KKTK) was detected in the pellet fraction even when using vesicles that contained PIP 2 (Fig.
As expected, given that the TK substitutions were sufficient to severely affect pelleting with PS vesicles, none of the Boi1-PH(KKTK) was seen to co-sediment with PS vesicles (Fig.
In small-budded cells, the KK, TK, and KKTK mutant proteins showed localization to the bud in only 18, 21, and 27% of the cells, respectively (compared to in 89% of the cells for wild-type Boi1-GFP) (Fig.
5A, bottom panel, lanes 5 and 6), consistent with the view that the KKTK mutant is more severely impaired in the ability to bind PIP 2 than are the KK and TK mutants.
We found that, in conditions in which PIP 2 stimulated proteolysis of most of the wild-type Boi1-PH, PIP 2 did not stimulate the proteolysis of the TK, KK, and KKTK versions of Boi1-PH (Fig.