Words similar to kh
Example sentences for: kh
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Cells grown on coverslips were washed briefly in a phosphate-buffered saline (PBS) solution (pH 7.4, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM KH 2 PO 4 ) and then either fixed in 100% methanol at -20°C for 1 minute or at room temperature in a 4% formaldehyde (PFA) solution freshly made from paraformaldehyde (Electron Microscopy Sciences, Ft.
Hebrew shuns initial v, f, kh, and final b, p, k .
Embryos were enzymatically dechorionated with Pronase and extensively washed in embryo medium prior to injection Embryo medium = 13 mM NaCl; 4.2 mM NaHCO 3 ; 0.54 mM KCl; 0.025 mM Na 2 HPO 4 ; 0.044 mM KH 2 PO 4 ; 1.3 mM CaCl 2 ; 1 mM MgSO 4 ). Embryos were injected and maintained for 14 hours post fertilization on a bed of 0.7% agarose in embryo medium.
In addition, several peptide binders showed the presence of the RGG box (Figure 2A) and one sequence was found that contained the KH motif (Figure 2B), both of which are known RNA-binding motifs [ 8 9 ] . The isolation of surrogates containing generic RNA binding motifs is not unexpected and probably results from enhanced binding and concomitant enrichment of these peptides during the panning process.
Between stimulations the tongue was rinsed with artificial saliva, which contained 2 mM NaCl, 5 mM KCl, 3 mM NaHCO3, 3 mM KHCO3, 0.25 mM CaCl2, 0.25 mM MgCl2, 0.12 mM K2HPO4, 0.12 mM KH2PO4, 1.8 mM HCl for pH 7. Because the precise content of mouse saliva is not published, we decided to use twice-diluted formula of the artificial saliva we have used in other species (monkey, pig, rat) [ 20 25 41 ] . It maintained the taste buds in good condition and gave us stable and reproducible nerve recordings for many hours.