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We also used recently developed and well defined mouse monoclonal antibodies CFK-E12 (E12) and CWK-F12 (F12), which were made against human ERβ amino acids 256-505 hormone binding domain lacking the F domain [ 18 ] . The antibodies were kindly donated by Dr. Benita S. Katzenellenbogen of the Department of Molecular and Integrative Physiology, University of Illinois College of Medicine, Urbana, Illinois.