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Example sentences for: k-

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DARWIN (version 2.0) was used to detect sequence-similar proteins within E. coli K-12 and in 20 additional microbial genomes [ 32] (P.
Previous work has shown that a significant percentage of mutations in p53 and K- ras 2 are localized to mutational hotspots, namely codons 175, 245, 248, 249, 273, and 282 in p53 and codons 12, 13, and 61 in K- ras 2 [reviewed in [ 14 17 18 ] ]. Each of these mutational hotspots was sequenced in the genomic DNA of various pancreatic cell lines using polony technology as follows.
The JNK pathway is involved in apoptosis [ 13 ] , arginine vasopressin-induced increases in smooth muscle α-actin in vascular smooth muscle cells [ 14 ] as well as in phosphorylation of transcription factors c-jun, ATF-2 and ELK-1 and phosphorylation of Na-K-2CI cotransporter [ 15 ] . The p38 pathway has been implicated in apoptosis [ 16 ] , neointimal hyperplasia after vascular injury [ 17 ] as well as angiotensin ll-induced contraction in vascular smooth muscle [ 18 ] .
A sequencing primer was hybridized to the single-stranded copy of the PCR-amplified p53/K- ras 2 fragment and a single base extension with either a Cy-3 or Cy-5 labeled dNTP was performed prior to scanning on a microarray scanner.
The genotypes of the E. coli K-12 strains, plasmids, and the bacteriophage used in this study are listed in Table 1and Figure 1. Strains were maintained on Luria broth or solid LB medium [ 32 ] . Ampicillin was added as needed at 100 mg/L.