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Example sentences for: jurkat
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However, others have shown that lower doses ≤ 100 μg/ml of SN50 specifically inhibited NF-κB nuclear translocation in human peripheral blood lymphocytes and murine T cells [ 33 63 ] . These reported differences are likely due to dose-dependent or cell-specific differences in the effect of SN50 [ 64 ] . Given that: (1) embryonic SMGs were cultured in the presence of 100 μg/ml SN50, (2) immunodetectable NF-κB was absent from SMG epithelia nuclei in TNF + SN50-treated explants, and (3) one cannot extrapolate observations in Jurkat cells to those in primary cells [ 64 ] or organ cultures, it is most probable that our observed interruption of SMG development is proximately due exclusively to the inhibition of NF-κB nuclear translocation.
Apoptosis was induced in Jurkat T cells (10 6/ml) by exposure to 1 μM staurosporine (STS) (ALEXIS, San Diego, CA, USA) for up to 4 hours.
It is well established that specific intracellular autoantigens are cleaved into different fragments in Jurkat T cells and other cell types undergoing apoptosis and necrosis [ 20 ] . Mancini et al . reported that golgin-160 (native protein, 160 kDa) was cleaved into a 140 kDa fragment during apoptosis [ 29 ] . We therefore performed an immunoblotting analysis using extracts from Jurkat cells treated with apoptosis-inducing reagent (1 μM STS) or necrosis-inducing reagent (0.
SK-N-BE neuroblastoma cells, Jurkat (TIB-152) and Jiyoye (CCL-87) cells were obtained from the ATCC (Manassas, VA).
Human Jurkat and HEp-2 cells were obtained from American Type Culture Collection (Rockville, MD, USA) and were cultured in RPMI 1640 and Dulbecco's modified Eagle's medium (Life Technologies, Rockville, MD, USA), respectively, containing 10% fetal bovine serum under standard conditions.
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