Words similar to jurkat
Example sentences for: jurkat
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Earlier studies have shown that blockade of caspase-8 activation in HeLa cells and deletion of caspase-8 in Jurkat cells prevented activation of downstream signaling, resulting in a failure of the cells to undergo apoptosis in response to TRAIL [ 46 ] . Similarly, another group has demonstrated that apoptosis induced by S. aureus in bovine mammary epithelial (MAC-T) cells utilizes a mechanism involving caspases-8 and -3, which are two key components of the proteolytic cascade that leads to apoptosis [ 30 ] . Results from the current study demonstrate a two-fold increase in caspase-8 activation upon infection with S. aureus (Fig.
In some experiments, Jurkat cells were incubated for 1 hour in the presence of the pancaspase inhibitor benzylocarbonyl-Val-Ala-Asp-fluromethyl-ketone (zVAD-fmk) (ALEXIS), used at 100 μM, prior to addition of STS.
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
SK-N-BE neuroblastoma cells, Jurkat (TIB-152) and Jiyoye (CCL-87) cells were obtained from the ATCC (Manassas, VA).
For example, in the Jurkat lymphoma cell line, which is deficient in the inositol phosphatase PTEN, Itk is constitutively associated with the membrane [ 36].
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