Words similar to jurkat

Example sentences for: jurkat

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In some experiments, Jurkat cells were incubated for 1 hour in the presence of the pancaspase inhibitor benzylocarbonyl-Val-Ala-Asp-fluromethyl-ketone (zVAD-fmk) (ALEXIS), used at 100 μM, prior to addition of STS.
The binding of TNFα to TNFR2 on intact cells was measured in a radioareceptor assay using Jurkat and Jiyoye cells and the MultiSreen™ assay filter plate system as described [ 39 ] . Data analysis was performed with an add-in for Microsoft Excel, Xlfit™ (version 2.0) from ID Business Solutions (Guildford, UK), which allows curve fitting using nonlinear regression.
Jurkat cells expressing SV40 large T antigen (Jurkat Tag) were a generous gift from Dr. Gerald Crabtree (Stanford) and were maintained in RPMI 1640 supplemented with 10% fetal calf serum, penicillin, streptomycin, glutamine, and 50 μM 2-mercaptoethanol.
It is known, for example, that S. aureus α-toxin induces apoptosis in Jurkat cells via the Bcl-2-controlled mitochondrial death pathway [ 32 ] ; therefore, neutralization of TRAIL would not be expected to interfere with this mechanism of apoptosis induction.
Apoptosis was induced in Jurkat T cells (10 6/ml) by exposure to 1 μM staurosporine (STS) (ALEXIS, San Diego, CA, USA) for up to 4 hours.