Words similar to jurkat
Example sentences for: jurkat
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After incubation in the presence of cell-death-inducing reagents, Jurkat cells were centrifuged at 200 × g for 30 min, followed by one wash at 1000 × g for 10 min in PBS containing Complete Protease Inhibitor cocktail (Roche, Mannheim, Germany).
However, others have shown that lower doses ≤ 100 μg/ml of SN50 specifically inhibited NF-κB nuclear translocation in human peripheral blood lymphocytes and murine T cells [ 33 63 ] . These reported differences are likely due to dose-dependent or cell-specific differences in the effect of SN50 [ 64 ] . Given that: (1) embryonic SMGs were cultured in the presence of 100 μg/ml SN50, (2) immunodetectable NF-κB was absent from SMG epithelia nuclei in TNF + SN50-treated explants, and (3) one cannot extrapolate observations in Jurkat cells to those in primary cells [ 64 ] or organ cultures, it is most probable that our observed interruption of SMG development is proximately due exclusively to the inhibition of NF-κB nuclear translocation.
None of the three weakly positive sera were positive using an immunoblot with apoptotic Jurkat cells, but two of the three sera positive by immunoblot were also positive using an immunoblot with apoptotic Jurkat cells.
In cases where discrepant results were obtained between immunoblot and EIA testing, sera were immunoblotted using a more sensitive technique against both intact and apoptotic Jurkat lysates, as previously described [ 18 19 ] using sera diluted 1:5000.
It is well established that specific intracellular autoantigens are cleaved into different fragments in Jurkat T cells and other cell types undergoing apoptosis and necrosis [ 20 ] . Mancini et al . reported that golgin-160 (native protein, 160 kDa) was cleaved into a 140 kDa fragment during apoptosis [ 29 ] . We therefore performed an immunoblotting analysis using extracts from Jurkat cells treated with apoptosis-inducing reagent (1 μM STS) or necrosis-inducing reagent (0.
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