Words similar to jurkat
Example sentences for: jurkat
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For example, the N- and C-terminal regions (amino acids 1-171 and 727-927, respectively) of murine NFATp function as activation domains when fused to a heterologous DNA binding domain in transient transfection assays in Jurkat cells [ 28].
Jurkat Tag cells were transfected by square wave electroporation using the parameters of 300 V and 10 ms.
1) first led us to investigate whether DEK in nuclear extracts binds to MHC class II Y-box regulatory elements in vitro . With a double-stranded oligonucleotide probe containing the HLA-DQA1*0101 Y-box sequence, EMSAs revealed similar binding activity in nuclear extracts from B lymphoid (Namalwa), T lymphoid (Jurkat, CEM), monocytoid (U937), and HeLa cell lines (Fig.
KcF12 inhibits TNFα binding to Jiyoye cells with a Ki of about 2 uM whereas the Ki for the peptide in Jurkat cells was found to be >30 uM (Figure 3).
Since TNFα binding to Jurkat probably involves both TNFR1 and TNFR2, the data offers additional confirmation of KcF12's selectivity for TNFR2.