Words similar to jolla

Example sentences for: jolla

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Northern blot analysis was carried out according to QuikHyb hybridization solution protocol (Stratagene, La Jolla, CA).
Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).
To establish cell lines stably expressing asbt, a cDNA encoding the hamster asbt [ 36 ] was cloned into pBK-CMV expression vector (Stratagene, La Jolla, CA).
A stable HeLa cell line expressing GFP-NFAT was generously provided by Larry Gerace (The Scripps Research Institute, La Jolla, CA).
; 4) to antagonize the effects of protein kinase C, the specific antagonist chelerythrine (ref 30; 10 nM, Calbiochem-Novabiochem Intl, La Jolla, CA) was added to the perfusate when inducing positive inotropy.