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Example sentences for: jiyoye
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The binding of TNFα to TNFR2 on intact cells was measured in a radioareceptor assay using Jurkat and Jiyoye cells and the MultiSreen™ assay filter plate system as described [ 39 ] . Data analysis was performed with an add-in for Microsoft Excel, Xlfit™ (version 2.0) from ID Business Solutions (Guildford, UK), which allows curve fitting using nonlinear regression.
Jurkat cells have been reported to have approximately equal numbers of TNFR1 and TNFR2 per cell [ 27 ] whereas Jiyoye cells express mainly TNFR2 (unpublished data).
KcF12 inhibits TNFα binding to Jiyoye cells with a Ki of about 2 uM whereas the Ki for the peptide in Jurkat cells was found to be >30 uM (Figure 3).
SK-N-BE neuroblastoma cells, Jurkat (TIB-152) and Jiyoye (CCL-87) cells were obtained from the ATCC (Manassas, VA).
SK-N-BE and Jurkat cells were maintained in RPMI 1640 media supplemented with 10% fetal bovine serum (FBS) and 2 mM GlutaMAX™-I, while Jiyoye cells were maintained in RPMI 1640 media supplemented with 15% FBS and 2 mM GlutaMAX™.