Words similar to jfl

Example sentences for: jfl

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In view of the mostly normal in vitro GTPase and assembly, it was remarkable that all lateral mutants failed to complement ftsZ84 . So far this failure to complement has only been tested in the JFL101(pBS58) system, but the reduced expression of the mutant proteins suggests a possible dominant negative effect.
These results also point to the limitations of the JFL101 (pBS58) system to characterize phenotypes of mutant ftsZ . This plasmid uses the endogenous ftsZ promoters, which are constitutively activated and may be subject to repression by second site mutations.
JFL101 carries the temperature sensitive ftsZ84 mutation at the genomic locus, and is unable to divide at 42°C, although it can grow and divide normally at 30°C.
(Data from the present study (JFL) were pooled for the purpose of this comparison.)
These mutants in pBS58 were tested for the ability to complement the ftsZ84 (ts) mutation of JFL101, supporting growth and division when grown in liquid LB containing 1% NaCl with 100 μg/mL spectinomycin.