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Example sentences for: javanica
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Sequences were obtained from the Parasitic Nematode Sequencing Project (PNSP) [ 30 ] including clustered Meloidogyne ESTs built with the NemaGene approach [ 28 ] . We analyzed 1,799 M. incognita (WMi) sequences and 3,119 M. javanica (WMj) sequences from these PNSP clusters.
To examine further the relationship between putative nodL candidates found in M. incognita and M. javanica with the cognate genes in rhizobia, we undertook a phylogenetic analysis and found that the two nematode genes fall squarely within the rhizobial nodL clade (Figure 2).
In C. elegans , two genes each encode unusual bifunctional enzymes containing both isocitrate lyase and malate synthase domains [ 49 ] . Since the isocitrate lyase domain lies within the amino-terminal half of the C. elegans bifunctional enzyme and none of the Meloidogyne EST reads stretches across both domains, further sequencing of the 3' end of cDNA clones from the M. hapla or M. javanica isocitrate lyase ESTs will be necessary to determine whether the Meloidogyne genus contains a bifunctional glyoxylate enzyme homolog similar to that of
The notable exception is Mj-CM , which is postulated to encode chorismate mutase in M. javanica [ 20 ] . To examine why this gene was not identified by our filtering process, we used both Mj-CM sequences found in GenBank (AF095949, AF095950) in a series of BLASTX queries.
Another RKN gene also postulated to have been acquired by HGT, and which encodes polyglutamate synthetase, was previously identified in M. artiellia [ 23 ] . Significantly, hybridization data showed that this particular gene is absent from both the M. javanica and G. rostochiensis genomes [ 23 ] . We speculate that acquisition of this gene by M. artiellia is a recent HGT event (event 'c', Figure 1), and thus it is truly absent from the Meloidogyne genomes from which our datasets were derived.
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