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Example sentences for: javanica
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Following clustering, comparative analyses were performed using WU-BLASTX and WU-TBLASTX [ 92 93 ] with 1,798 contig consensus sequences (themselves grouped into 1,625 cluster groups) as queries versus multiple databases including SWIR v.21 (5/19/2000) non-redundant protein database and Wormpep v.54 C. elegans protein database (Wellcome Trust Sanger Institute, unpublished work), C. elegans mitochondrial protein sequences, and six internally constructed databases using intersections of data from the GenBank nucleotide database and dbEST [ 96 ] . These include: nemnoele (all nucleotide data from the phylum Nematoda with C. elegans removed); nemnoelenomi (nemnoele with M. incognita removed); nemnoelenomel (nemnoele with all Meloidogyne species removed); nemnoelenotyl (nemnoele with all Tylenchida species removed); yestylnomel (all Tylenchida species except Meloidogyne ); mj (only M. javanica sequences).
The notable exception is Mj-CM , which is postulated to encode chorismate mutase in M. javanica [ 20 ] . To examine why this gene was not identified by our filtering process, we used both Mj-CM sequences found in GenBank (AF095949, AF095950) in a series of BLASTX queries.
Included are 32,735 sequences from Meloidogyne species ( M. incognita 12,752, M. hapla 11,049, M. javanica 5,600, M. arenaria 3,334), as well as ESTs from cyst nematode species ( G. rostochiensis 5,934, H. glycines 4,327, G. pallida 1,832), and the lesion nematode ( P. penetrans 2,048).
Using clustered ESTs ( M. incognita and M. javanica sequences, named WMi and WMj) as queries to the worm, fly and bacterial protein databases (which are based on gene annotation) produced a similar degree of reduction (Table 1).
MI00244.cl, for example, had homology to 47 ESTs in our collection from other Tylenchida species including root-knot nematodes M. javanica, M. hapla and M. arenaria , cyst nematodes H. glycines and G. rostochiensis , and the lesion nematode Pratylenchus penetrans with E-values from 7e-78 to 3e-05.
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