Words similar to iptg
Example sentences for: iptg
How can you use “iptg” in a sentence? Here are some example sentences to help you improve your vocabulary:
GST-fusions were expressed in E. Coli BL21 [ 28 ] . Cultures were grown to OD600 1.5 at room temperatures (approximately 22°C) and protein production was initiated by addition of IPTG to 1 mM.
After 2 hours, isopropyl thio-β-D-galactoside (IPTG) was added to a final concentration of 1 mM to induce gene expression and the cultures.
These initial studies showed that the wild-type autogene did not affect cellular viability for at least 2-3 hours after induction with IPTG.
Inhibition of chimeric GFP-LacI/NLS protein binding in situto LacOsby IPTG
In order to evaluate the effect of toxicity of autogene on the selection process, the effect of the wild-type autogene construct (pET/T7p/T7) on cell growth was studied before and after induction with IPTG (Figure 3).