Example sentences for: iptg

How can you use “iptg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After IPTG induction (100 μM, 1 hour, 37°C) and plating on ampicillin (100 μg/ml) ± tetracycline (25 μg/ml) plates, mobility events were identified as amp R tet R colonies.

  • GST-p35-C/EBPβ, GST-p20-C/EBPβ, or GST alone was produced in BL21 E. Coli by 80 mM isopropyl-β-D-thiogalactopyranoside (IPTG) induction overnight at 37°C.

  • Introduction of the plasmids into E. coli BL21(DE3) cells containing the compatible RIG plasmid [ 39 ] , induction of the proteins with IPTG, and purification through Ni-chelation chromatography were performed using the His-Bind kit reagents and procedure (Novagen).

  • 3,000) were lifted to IPTG plates and screened for their inability to thrive.

  • If the binding is specific to the LacOs inserted in the genome, the observed fluorescence spots should be suppressed by the addition of IPTG.

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