Example sentences for: iptg

How can you use “iptg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.

  • The transformation mix was plated onto several LB-AMP plates containing Xgal and isopropyl β-D thiogalactoside (IPTG).

  • Protein induction was performed using isopropyl-β-D-thiogalactopyranoside (IPTG) as described by Novagen Inc.

  • Therefore, the fraction of colonies that were lost on replica plating to IPTG was assumed to be proportional to the accumulation of active autogene variants.

  • These initial studies showed that the wild-type autogene did not affect cellular viability for at least 2-3 hours after induction with IPTG.

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