Words similar to iodide
Example sentences for: iodide
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Flow cytometry was also used to assess GC viability and size, using methods described previously in detail to determine propidium iodide uptake and forward (small angle light) scatter, respectively [ 18 25 ] . Briefly, ten thousand cells were examined per sample using a Becton Dickinson FACSCalibur to excite the cells with a 488 nM argon laser, and the collected data obtained were analyzed using Cell Quest™ software (Becton Dickinson, Mount View, CA).
These data provide additional evidence that 4-AP exerts neither an anti-proliferative nor a pro-apoptotic influence on serum-supplemented GC cultures, because PCNA expression is a sensitive marker of GC proliferation and apoptosis [ 18 26 27 ] . The lack of 4-AP effect on GC viability is also evident from the flow cytometric analysis; the percentages of cells staining positive for propidium iodide were 8 ± 2% and 7 ± 2% in the presence and absence of drug (n = 4 samples of 10,000 cells/each from 4 GC isolations).
The harvested cells (typically 5 × 10 6to 1 × 10 7cells) were resuspended in 300 μl of 8 μg/ml propidium iodide (Molecular Probes) in PBS containing 0.1% NP-40 and 10 μg/ml RNAse A, then incubated at room temperature in the dark for 30 minutes.
In situ detection of DNA fragmentation was performed by incorporation of fluorescein-12-dUTP at the 3'-OH ends of DNA using Terminal deoxynucleotidyl Transferase (TUNEL assay) (Apoptosis Detection System, Fluorescein, Promega, Madison, WI), as performed previously by this laboratory [ 7 26 ] . Nuclei were counterstained using propidium iodide (Sigma Chemical Co., St. Louis, MO).
This assay uses propidium iodide (PI) dye to differentiate live and dead cell [ 26 27 28 ] . Cells that have lost membrane integrity cannot exclude PI, which emits a red fluorescence after binding to cellular DNA or double stranded RNA.
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