Words similar to invitrogen

Example sentences for: invitrogen

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Following cell thaw, NSs were expanded in suspension culture, and in each case were used between passages 5 and 7. Undifferentiated human NS cells (stage 1) were cultured and expanded in medium containing X-VIVO15 (Cambrex, Walkersville, Maryland, United States), N2 supplement (Invitrogen, Carlsbad, California, United States), 2 μg/ml heparin, 10 ng/ml leukemia inhibitory factor (Chemicon, Temecula, California, United States), 20 ng/ml epidermal growth factor (R&D Systems, Minneapolis, Minnesota, United States), and 20 ng/ml fibroblast growth factor-2 (R&D Systems), and they were frozen after enzymatic dissociation.
At each time point, cells were lysed directly in the culture dishes for mRNA isolation using oligo(dT) binding buffer (Invitrogen FastTrack 2.0) containing proteinases for ribonuclease degradation (see below).
Oligonucleotides were synthesized at a 50 nmol scale by Invitrogen/Life Technologies (Carlsbad, CA) or Operon Technologies (Alameda, CA), and resuspended in sterile milliQ water to a final concentration of 100 μM.
Protein-bound oligonucleotides from the fifth round of selection were purified and subcloned into plasmid vectors pCR 2.1 (Invitrogen, Carlsbad, CA).
G418-resistant cell lines were selected in DMEM + 10% fetal bovine serum (Clontech, Tet-free) + 200 μg/ml Geneticin (Invitrogen).