Words similar to invitrogen
Example sentences for: invitrogen
How can you use “invitrogen” in a sentence? Here are some example sentences to help you improve your vocabulary:
These fragments were subsequently cloned into the PCRII vector (TA cloning Kit, Invitrogen) and the identity of each was confirmed by sequencing in both directions.
Human TTP cDNA was generated by RT-PCR from THP-1 RNA and cloned into the pcDNA 3.1His-C vector (Invitrogen, Carlsbad, CA, USA), which encodes a histidine and epitope (EXPRESS-TAG) at the N-terminus of the protein.
PCR products were either purified using ExoSAP-IT (USB Co, Cleveland, OH) or subcloned into a TA cloning vector (Invitrogen, Carlsbad, CA) per the manufacturer's instructions.
5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).
Total RNA was prepared by using TRIZOL (Invitrogen) and RQ1 RNase-free DNase (Promega, Madison, Wisconsin, United States).
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