Example sentences for: invitrogen

How can you use “invitrogen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were dissociated in 50 ml tubes by enzymatic digestion with 0.25% Collagenase (Worthington Biochemical, Collagenase Type 2 - CLS 2) and 0.25% Trypsin/EDTA (Invitrogen/Gibco BRL) for 1 hour at 37°C, or until the soft tissues were floating in the solution.

  • At the beginning of each experiment, chondrocytes or OA synovial fibroblasts were washed three times with Hanks balanced salt solution (Cellgro) to remove traces of serum, and placed in DMEM containing 0.2% lactalbumin hydrolysate (Invitrogen, Carlsbad, CA, USA).

  • This PCR product was subsequently used as template DNA in a labeling reaction using the Radprime DNA labeling system (Invitrogen, Carlsbad, CA).

  • Total RNA was extracted from human breast cell lines, frozen breast tumor tissues and normal breast tissues with Trizol reagent (Invitrogen, Gaithersburg, MD, USA) in accordance with the manufacturer's protocol.

  • 5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).

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