Words similar to intragenic
Example sentences for: intragenic
How can you use “intragenic” in a sentence? Here are some example sentences to help you improve your vocabulary:
For example, Panc-1 was determined to possess only one copy of p53, which possessed an intragenic mutation in codon 273, and two copies of K- ras 2, one wildtype and one with an intragenic mutation in codon 12.
Mating, meiosis, and preparation of free spores were as described [ 54 55 65 ] . Intergenic and intragenic recombinant frequencies were determined as described [ 38 65 ] . Because diploid spores could contain complementing markers and be mistaken for recombinants, we tested all spore colonies for diploidy by replica plating on complete media containing Phloxin-B (YEA-B).
Direct sequencing of these products confirmed both of these contained Abcg8 rat cDNA sequences, but the larger product contained a 66 bp insertion, resulting in a predicted in-frame addition of 22 amino acids, between exons 2 and 3. To confirm that this insertion resulted from an alternatively spliced mRNA, we amplified and sequenced the intragenic region between exons 2 and 3 and identified a 66 bp exon (exon 2b), flanked by canonical splice-sites (see Table 2and Fig.
M6P/IGF2R loss of heterozygosity occurs frequently in human breast, liver and lung cancer [ 6 20 21 22 ] , and the remaining allele of 30 to 50% of these tumors contains an intragenic loss-of-function point mutation in the ligand binding domains [ 35 ] . The M6P/IGF2R is also commonly mutated in gastrointestinal and endometrial malignancies because its coding sequence contains a poly-G region that is a mutational target in tumors with mismatch repair deficiencies and microsatellite instability [ 23 24 ] . Functional studies show that the introduction of an exogenous wild-type M6P/IGF2R into human colorectal cancer cells with a single inactivated allele significantly decreases growth rate and enhances apoptosis [ 26 ] . Conversely, loss of M6P/IGF2R expression promotes cancer cell growth by increasing intracellular signaling from both the insulin-like growth factor I receptor and the insulin receptors [ 36 ] .
We have shown that polony technology is an improved method to study, and potentially diagnosis, cancer [ 22 ] . Specifically, polony technology was successfully applied to both detect intragenic mutations in well-defined mutational hotspots in key cancer genes as well as determine if loss of heterozygosity of these same genes had occurred.
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