Words similar to insulin-treated
Example sentences for: insulin-treated
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Rapamycin also down regulated the Thr421/Ser424 phosphorylation in p70 S6 kinase in control and insulin-treated cells at both time-points (Fig.
PD98059 did not affect Thr389 or Thr421/Ser424 phosphorylation in p70 S6 kinase in insulin-treated cells at either time point, but slightly decreased their phosphorylation when added alone (Figs.
The total amount of p44/42 MAPK was equal and remained level in control and insulin-treated cells at all time-points (Fig.
In cultured human muscle cells, only the PI 3-kinase - p70 S6 kinase pathway, and not the MAPK pathway, mediates insulin up-regulation of p85α PI 3-kinase gene expression [ 26 ] . Similarly, in L6 myotubes, insulin induces hexokinase II gene transcription via the rapamycin-sensitive PI 3-kinase pathway, but not via the MAPK pathway [ 27 ] . The PI 3-kinase - p70 S6 kinase pathway, but not the MAPK pathway, mediates insulin induction of glucose-6-phosphate dehydrogenase gene expression in primary rat hepatocytes [ 28 ] . In H411E liver cells, insulin down-regulates cAMP-induced phosphoenolpyruvate carboxykinase gene transcription via the PI 3-kinase pathway, but not via the MAPK pathway [ 29 ] . Phosphorylated p70 S6 kinase has been shown to be present in the nucleus in insulin-treated cells [ 30 ] . Moreover, it has been shown that p70 S6 kinase phosphorylates and activates members of the cAMP response element modulator family of transcription factors [ 31 ] . These data raise the possibility that transcriptional regulation of SP-A gene expression occurs via the PI 3-kinase - p70 S6 kinase pathway.
The intensity of the immunoreactive bands of phospho-p44/42 MAPK from insulin-treated cells was 0.92 ± 0.10 relative to controls, which were made equal to one (data are the mean of two experiments performed in duplicate plus or minus the standard deviation of the mean).
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