Example sentences for: inframe

How can you use “inframe” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • GST-RANKL was purified as described previously [ 32 ] . Briefly, a fragment of murine RANKL cDNA was cloned inframe into the pGEX-4T vector (Amersham Pharmacia, Piscataway, USA) and expressed in BL21 bacteria (Amersham Pharmacia) induced with 0.1 mM IPTG (Gibco) for 5 hours at 30°C.

  • For the yeast two-hybrid screen, full-length Smad1 was subcloned into BamH1/Not1 sites of PEG202 by a PCR method, resulting in the inframe fusion between the LexA DNA binding domain (1-202) and Smad1.


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