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Example sentences for: including
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Glaucoma is a leading cause of blindness that involves loss of retinal ganglion cells and degeneration of the optic nerve [ 1 ] . Glaucoma is usually associated with high intraocular pressure (IOP) that results from an increased resistance to drainage of the ocular fluid [ 1 ] . Developmental disorders of the ocular anterior segment, including Axenfeld-Rieger syndrome, are often associated with elevated IOP and glaucoma [ 1 2 ] . The developmental sequence and structure of the human and mouse ocular drainage structures are similar [ 3 ] , and the same genes are known to cause anterior segment dysgenesis (ASD) in humans and mice.
A variety of amino-terminal fusions maintain enzymatic activity (or produce enzymatically active breakdown products) including those fused to codon 12 of NPT [ 48 49 50 51 52 ] . Moreover, selection of resistant cell clones requires only minimal levels of Neo gene expression [ 53 ] . Still, genes providing the appropriate introns are expected to provide larger targets for gene trap mutagenesis than genes lacking such introns.
Several variations of BPCR with Taq polymerase were carried out (data not shown), including: 1) cut and intact templates were prepared with primers b1 and b2, 2) purified ssDNA instead of phage particles was used as a reference template and 3) cut and intact templates were prepared with the Stoffel fragment of Taq polymerase.
Concentrated recombinant retrovirus, generated by super-speed centrifugation of retroviral supernatant, has been used to improve the transduction efficiency of primary cells, including hepatocytes [ 9 ] and endothelial cells [ 11 ] . In these prior reports, concentration was accomplished by centrifugation for 16 hours at a RCF of 6000 g . We used a recombinant retrovirus encoding the green fluorescent protein to optimize a protocol to rapidly and efficiently concentrate retrovirus by superspeed centrifugation.
Laminins are major components of the extracellular matrix and exist in many isoforms with varying functions including cell adhesion, neurite outgrowth, and cellular signaling [ 7 8 9 10 ] . Laminin binds agrin [ 11 ] , and colocalizes with AChR aggregates [ 12 13 14 ] . It also binds with α-Dystroglycan (α-DG), an important part of the dystrophin/utrophin glycoprotein complex (Reviewed in [ 15 16 ] ). The dystrophin complex is also found concentrated at the neuromuscular junction with AChRs and laminin, and for this and other reasons is thought to be involved in both formation and maintenance of the NMJ [ 2 17 18 19 20 21 22 23 ] .
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