Words similar to in-frame
Example sentences for: in-frame
How can you use “in-frame” in a sentence? Here are some example sentences to help you improve your vocabulary:
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
The GAL4 fusion plasmids were obtained by subcloning the appropriate cDNAs into the pGaL4PolyII plasmid [ 24 ] , in-frame with the yeast GAL4 binding domain coding sequence.
Srf I restriction digestion was then used to remove the Kan r cassette from the clones carrying in-frame insertions, thereby creating a sequence encoding a full-length fusion protein.
This prediction is based on the occurrence of three in-frame stop codons upstream of the indicated ATG, within the 242 nt 5'-untranslated region (5'-UTR) (see Additional File 1).
Direct sequencing of these products confirmed both of these contained Abcg8 rat cDNA sequences, but the larger product contained a 66 bp insertion, resulting in a predicted in-frame addition of 22 amino acids, between exons 2 and 3. To confirm that this insertion resulted from an alternatively spliced mRNA, we amplified and sequenced the intragenic region between exons 2 and 3 and identified a 66 bp exon (exon 2b), flanked by canonical splice-sites (see Table 2and Fig.