Words similar to in-frame

Example sentences for: in-frame

How can you use “in-frame” in a sentence? Here are some example sentences to help you improve your vocabulary:

Two separate transpositions with the different colored transposons, followed by co-transfections in the visual screen (one potential green clone and one potential cyan clone per well), can identify twice as many in-frame insertions in a given number of transfections.
Each clone containing an in-frame insertion should encode a truncated α s protein with GFP at the carboxy-terminus due to a stop codon in the Kan r . Thirty-five of the PCR-positive clones were transiently expressed in HEK 293 cells, and 13 were fluorescent.
The insert of a Dazap1 cDNA clone P21, which encoded the C-terminal portion of DAZAP1 (starting from aa #197), was cloned in-frame into the Eco RI/ Xho I sites of an expression vector pET32b (Novagen, Madison, WI).
The discovery that all of the in-frame insertions in α s produced truncated fluorescent fusion proteins suggested that we could identify in frame insertions by transiently expressing all of the transposed clones and visually screening them for fluorescence.
Even though the library was biased at the DNA level to avoid stop codons (see Methods), roughly one third of the clones were expected to contain in-frame stop codons within the insert, thus producing a truncated GFP molecule containing only the first 157 amino acids of GFP followed by C-terminal peptides of variable length up to 15 amino acids.