Example sentences for: immuno-fluorescence

How can you use “immuno-fluorescence” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Experiments using immuno-fluorescence in vertebrate cells are beginning to order the recruitment and timing of incorporation of individual nucleoporins during post-mitotic re-assembly [ 12 13 14 15 ] . Additionally, in vitro assembly studies with Xenopus egg or mitotic cell extracts have revealed several intermediates in NPC assembly and demonstrated a requirement for an intact double nuclear membrane prior to post-mitotic NPC assembly [ 16 17 18 19 ] . The modular nature of the NPC structure has also suggested that subcomplexes of discrete Nups may initially form and provide building blocks for biogenesis.

  • Indirect immuno-fluorescence was performed as previously described [ 46 ] . Rabbit anti-Kar2 polyclonal antibodies [ 45 ] were used at 1:5000 and detected with Texas Red Goat-anti-Rabbit secondary antibody.

  • Similar results were obtained when other cell lines (including HeLa, NIH3T3, and CHO cell lines) were used or immuno-fluorescence studies were carried out with FLAG-COP1 instead of GFP-COP1 (data not shown).

  • Endogenous COP1 is not detectable by immuno-fluorescence with our anti-COP1 antibodies, probably due to a very low expression level.

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