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Example sentences for: img
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Small intensely fluorescent (SIF) cells, intensely stained with TH, showed little to moderate MIF staining in the IMG (Fig.
Sections from the MPG, the IMG and the sympathetic chain were processed for double-immunofluorescence in order to visualize both MIF and tyrosine hydroxylase, as follows: Sections were exposed to 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Tx-100; 30 min) followed by incubation with anti-MIF (1:200) and anti-tyrosine hydroxylase (sheep polyclonal antibody; 1:1000; Pel-Freez; Rogers, AR) overnight at 4°C.
The following tissues were removed and placed in fixative at least overnight: bladder, ventral lobes of the prostate (in the males), both MPG, the inferior mesenteric ganglia (IMG), the sympathetic chain (L3-L5),