Example sentences for: ii-iii

How can you use “ii-iii” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The present studies show EC coupling recovery by a frame-shift mutant of α 1S due to protein-protein complementation of the N-terminal and C-terminal halves of α 1S . The N-terminal half houses repeats I and II with the adjoining cytosolic loop and the C-terminal half houses most of the II-III loop, along with repeats III and IV with the adjoining loop.

  • The HindIII-XhoI fragment (nt 2228 to nt 2878) encompasing the II-III loop was subcloned into pCR 2.1 TOPO TA (Invitrogen, CA) and this plasmid was further used for PCR reactions.

  • Earlier studies in the voltage-gated Na +channel had shown that pore function was not compromised when the II-III linker or III-IV linker was cut and the two fragments were coexpressed each in a separate vector [ 37 ] . We would thus conclude that in the case of the Ca 2+channel, an intact II-III loop is essential for this function since neither fs-α 1S nor the combined expression of the two truncated fragments (not shown) was able to rescue Ca 2+current.

  • 6Cshows that the II-III loop antibody recognized the C-terminus half when cells were transfected with fs-α 1S but not when myotubes were transfected with fs-α 1S M701I.

  • The C-terminal fragment was identified with SKI, a rabbit polyclonal antibody against the II-III loop of α 1S (Ala739-Ile752) previously characterized [ 34 ] . Cells were scrapped from tissue cultures dishes with cold PBS plus protease inhibitors and spun in a cold table-top centrifuge.

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