Words similar to ii-iii

Example sentences for: ii-iii

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The HindIII-XhoI fragment (nt 2228 to nt 2878) encompasing the II-III loop was subcloned into pCR 2.1 TOPO TA (Invitrogen, CA) and this plasmid was further used for PCR reactions.
The C-terminal fragment was identified with SKI, a rabbit polyclonal antibody against the II-III loop of α 1S (Ala739-Ile752) previously characterized [ 34 ] . Cells were scrapped from tissue cultures dishes with cold PBS plus protease inhibitors and spun in a cold table-top centrifuge.
This result is entirely consistent with the identification of the II-III loop as critical for enhancement of L-type Ca 2+current expression by the RyR1 [ 38 ] . However EC coupling, per se, can clearly proceed with a cut in the II-III loop.
This region was identified using chimeras of α 1S and α 1C [ 16 ] . Since Csk53 is present in α 1S Δ1-700 and was detected by the II-III loop antibody which is directed against the center portion of Csk53 [ 34 ] , the participation of this domain in the EC cannot be ruled-out.
The present studies show EC coupling recovery by a frame-shift mutant of α 1S due to protein-protein complementation of the N-terminal and C-terminal halves of α 1S . The N-terminal half houses repeats I and II with the adjoining cytosolic loop and the C-terminal half houses most of the II-III loop, along with repeats III and IV with the adjoining loop.