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One of the main advantages of SSH is that it normalizes the cDNA abundance so that cDNAs encoded by genes that are expressed infrequently, but nonetheless differentially, can be identified readily [ 33 ] . The efficiency of normalization is illustrated in Figure 2. The more uniform distribution of hybridization intensities obtained using the subtracted cDNA probe (Figure 2c) reflects the equalization in the concentrations of individual species present at markedly different concentrations in the initial unsubtracted cDNA populations (Figure 2b).
No statistically significant differences in accuracy of prognosis prediction were identified for the three scales assessed.
The strength of the putative interaction at Y142 can be identified by examining the change in binding of lerisetron as a result of the Y142A mutation.
We identified two known duplications from this analysis, which is in agreement with previous segmental duplication analyses available from the UCSC Genome Browser [ 14 16 ] . The first is derived from the a duplication of the CHRNA7 gene, characterized in detail by Riley et al . [ 17 ] . This sequence was duplicated from the intact CHRNA7 locus in 15q13.
We found that the pairwise survival method was indeed highly specific, with 97% of changes identified by this method also detected by t-test.