Words similar to ibmx
Example sentences for: ibmx
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Total post-Ficoll un-fractionated thymocytes were incubated in the presence or the absence of 10 nM PMA and/or 0.1 mM cAMP + 1 mM of the phosphodiesterase inhibitor isobutylemthylxnthine (IBMX).
4-aminopyridine (4-AP); analysis of variance (ANOVA); 8-(4-chlorophenylthio)adenosine-3',5'-cyclic monophosphorothioate (8-CPT-cAMP); 4',4' diisothicyanato-stilbene-2-2'-disulfonic acid (DIDS); bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC 4 (3)); 5α-dihydrotestosterone (5α-DHT); Dulbecco's modified eagle's medium (DMEM); enhanced chemiluminescence (ECL); ethylenediaminetetraacetic acid (EDTA); fetal bovine serum (FBS); follicle stimulating hormone (FSH); granulosa cells (GC); human chorionic gonadotropin (hCG); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES); 3β-hydroxysteroid dehydrogenase (3β-HSD); 3-isobutyl-1-methylxanthine (IBMX); least significant difference (LSD); luteinizing hormone (LH); mitogen activated protein kinase (MAPK); 1,3-Benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis [(acetyloxy)methyl] ester (PBFI-AM); phosphate-buffered saline (PBS); proliferating cell nuclear antigen (PCNA); protein kinase A (PKA); sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); steroidogenic acute regulatory protein (StAR); tris-buffered saline (TBS).
The phorbol esters 12-tetradecanoylphorbol-13 acetate (PMA), 4x-phorbol, 12,13 didecanoate (αPD2), 4B-phorbol 12,13 didecanoate (βPD2), the phorbol 12,13 dibutyrate (PDB), dibutyryl cyclic AMP, and the IBMX were obtained from Sigma Chemical Co. (St.
On the other hand, apoptosis was ruled out as a mechanism to explain the differential down-regulation of CD4 and CD8 by PMA and cAMP, on the grounds of two independent observations: (a) After a maximum of 4.0 hr incubation with either PMA or cAMP+ IBMX the cell viability was the same as the untreated controls (usually between 90-95%); (b) Treatment with dexamethasone, which is a known pro-apoptotic molecule did not have any effects on the expression of either CD4 or CD8 (data not shown) and; (c) As demonstrated earlier in the murine system, TCR engagement of CD4 +CD8 +induces positive selection but not apoptosis [ 12 ]
The medium was replaced 24 hours later and incubated one additional hour at 37°C in 450 μL DMEM plus 0.25 mM 3-isobutyl-1-methylxanthine (IBMX), and 40 mM indomethacin.