Example sentences for: i-pedf

How can you use “i-pedf” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Similar results were obtained when the cell-bound 125I-PEDF was separated by centrifugation, rather than filtration through glass-fiber filters, and comparing reactions with CM versus those with 1% BSA in PBS (data not shown).

  • Media conditioned by retinoblastoma cells enhances the 125I-PEDF binding to cell-surface receptors

  • We found that 22% of 125I-PEDF was retained by the membrane in the presence of concentrated CM, compared to only 4% retention in the presence of defined medium (non-conditioned medium) concentrated in an identical fashion.

  • Note that with the binding method used, the amount of 125I-PEDF retained in CM without cells is the same to the amount of non-specific 125I-PEDF binding (reactions with cells and in the presence of 50-fold molar excess of unlabeled PEDF) [ 18 ] , indicating retention by PEI-treated glass-fiber filters of PEDF-receptor or GAG-PEDF-receptor complexes rather than PEDF-GAG.

  • PEDF binding to cell-surface receptors was assayed using biologically active radioligand 125I-PEDF and Y-79 cells [ 18 ] by a widely-used method with a mechanism of retention of receptors on polyethylenimine-treated glass-fiber filters based mainly on ionic interactions [ 32 ] . Polyethylenimine binds strongly to glass, which is negatively charged and integral membrane proteins tend to be acidic.


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