Words similar to i-pedf
Example sentences for: i-pedf
How can you use “i-pedf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Note that with the binding method used, the amount of 125I-PEDF retained in CM without cells is the same to the amount of non-specific 125I-PEDF binding (reactions with cells and in the presence of 50-fold molar excess of unlabeled PEDF) [ 18 ] , indicating retention by PEI-treated glass-fiber filters of PEDF-receptor or GAG-PEDF-receptor complexes rather than PEDF-GAG.
Similar results were obtained when the cell-bound 125I-PEDF was separated by centrifugation, rather than filtration through glass-fiber filters, and comparing reactions with CM versus those with 1% BSA in PBS (data not shown).
We found that 22% of 125I-PEDF was retained by the membrane in the presence of concentrated CM, compared to only 4% retention in the presence of defined medium (non-conditioned medium) concentrated in an identical fashion.
We have demonstrated previously that biologically active 125I-PEDF binds specifically, competitively and with high affinity to cell-surface receptors of Y-79 cells [ 18 ] . Because the reaction conditions were identical to those used for biological assays, the binding reactions were performed in the presence of media conditioned by the cells for 16 hours (CM).
PEDF binding to cell-surface receptors was assayed using biologically active radioligand 125I-PEDF and Y-79 cells [ 18 ] by a widely-used method with a mechanism of retention of receptors on polyethylenimine-treated glass-fiber filters based mainly on ionic interactions [ 32 ] . Polyethylenimine binds strongly to glass, which is negatively charged and integral membrane proteins tend to be acidic.
Loading...