Example sentences for: i-pedf

How can you use “i-pedf” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PEDF binding to cell-surface receptors was assayed using biologically active radioligand 125I-PEDF and Y-79 cells [ 18 ] by a widely-used method with a mechanism of retention of receptors on polyethylenimine-treated glass-fiber filters based mainly on ionic interactions [ 32 ] . Polyethylenimine binds strongly to glass, which is negatively charged and integral membrane proteins tend to be acidic.

  • Effect of GAG lyases and chlorate on 125I-PEDF binding to cell-surface receptors

  • We have demonstrated previously that biologically active 125I-PEDF binds specifically, competitively and with high affinity to cell-surface receptors of Y-79 cells [ 18 ] . Because the reaction conditions were identical to those used for biological assays, the binding reactions were performed in the presence of media conditioned by the cells for 16 hours (CM).

  • Cells pretreated with sodium chlorate did not show changes in viability or morphology; however, the treatment resulted in a decrease in the specific 125I-PEDF binding to about 35% relative to untreated controls (Fig.

  • Note that with the binding method used, the amount of 125I-PEDF retained in CM without cells is the same to the amount of non-specific 125I-PEDF binding (reactions with cells and in the presence of 50-fold molar excess of unlabeled PEDF) [ 18 ] , indicating retention by PEI-treated glass-fiber filters of PEDF-receptor or GAG-PEDF-receptor complexes rather than PEDF-GAG.

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