Words similar to i-pedf

Example sentences for: i-pedf

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Note that with the binding method used, the amount of 125I-PEDF retained in CM without cells is the same to the amount of non-specific 125I-PEDF binding (reactions with cells and in the presence of 50-fold molar excess of unlabeled PEDF) [ 18 ] , indicating retention by PEI-treated glass-fiber filters of PEDF-receptor or GAG-PEDF-receptor complexes rather than PEDF-GAG.
The free and bound 125I-PEDF were separated by filtration through glass-fiber filters and the bound radioactivity was determined in the filters using a β-scintillation counter (Beckman, model LS 3801).
We have demonstrated previously that biologically active 125I-PEDF binds specifically, competitively and with high affinity to cell-surface receptors of Y-79 cells [ 18 ] . Because the reaction conditions were identical to those used for biological assays, the binding reactions were performed in the presence of media conditioned by the cells for 16 hours (CM).
Cells pretreated with sodium chlorate did not show changes in viability or morphology; however, the treatment resulted in a decrease in the specific 125I-PEDF binding to about 35% relative to untreated controls (Fig.
Complex formation between PEDF and CM components was assayed by a method using ultrafiltration through membranes of 100,000 MW exclusion limit [ 14 ] . Binding reactions were performed with a given concentration of 125I-PEDF in defined or conditioned media, and incubations with gentle rotation at 4°C for 2 h.