Words similar to i-pedf

Example sentences for: i-pedf

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Similar results were obtained when the cell-bound 125I-PEDF was separated by centrifugation, rather than filtration through glass-fiber filters, and comparing reactions with CM versus those with 1% BSA in PBS (data not shown).
Note that with the binding method used, the amount of 125I-PEDF retained in CM without cells is the same to the amount of non-specific 125I-PEDF binding (reactions with cells and in the presence of 50-fold molar excess of unlabeled PEDF) [ 18 ] , indicating retention by PEI-treated glass-fiber filters of PEDF-receptor or GAG-PEDF-receptor complexes rather than PEDF-GAG.
The free and bound 125I-PEDF were separated by filtration through glass-fiber filters and the bound radioactivity was determined in the filters using a β-scintillation counter (Beckman, model LS 3801).
Complex formation between PEDF and CM components was assayed by a method using ultrafiltration through membranes of 100,000 MW exclusion limit [ 14 ] . Binding reactions were performed with a given concentration of 125I-PEDF in defined or conditioned media, and incubations with gentle rotation at 4°C for 2 h.
Cells pretreated with sodium chlorate did not show changes in viability or morphology; however, the treatment resulted in a decrease in the specific 125I-PEDF binding to about 35% relative to untreated controls (Fig.