Example sentences for: hygromycin

How can you use “hygromycin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).

  • 4. Function of the terminator was determined by patching hygromycin B-resistant transformants LB medium containing kanamycin.

  • The entire mixture was plated on 117 150 mm LB agar plates containing hygromycin B. About 117,000 transformants were collected and pooled by flooding each plate with 2 ml LB and scraping the colonies off the plate.

  • Selection with hygromycin was begun 48 hr later.

  • Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).


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