Words similar to hydrated

Example sentences for: hydrated

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Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.
Yeast ORFs were amplified from a set of 6,218 plasmids using universal primers as described [ 1 ] . This set of ORFs was printed on separate slides, hydrated, and snap-dried as described [ 1 ] . Mixed Cy5/Cy3-labeled probes were hybridized to microarrays for 12-15 h at 60°C.
Paraffin blocks were cut in 5-6 μm sections, deparaffinized and hydrated to water prior to staining.