Words similar to humidified

Example sentences for: humidified

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The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.
Sections were hybridized for 16 h at 58-60 C in a humidified chamber.
The primary and secondary antibodies were incubated in a humidified chamber at 37°C for 30 minutes.
Freshly isolated GC were plated in the basic serum-supplemented culture media on collagen-coated 24-well (500 μl/well) or 6-well (2 ml/well) culture dishes, and incubated at 37° C in a humidified atmosphere of 5% CO 2 and air.
Microglia were characterized as described earlier [ 7 ] . Depending on the particular experimental protocol (see below), microglia averaging > than 95% viability were plated in either 60 mm × 15 mm polystyrene cell culture dishes, 96-well cell culture clusters, or 24-well cell culture clusters, with DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S, and placed in a humidified 5% CO 2 incubator at 36°C 18-24 hours prior to the experiments.