Example sentences for: humidified

How can you use “humidified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The primary and secondary antibodies were incubated in a humidified chamber at 37°C for 30 minutes.

  • Human prostate carcinoma cells, LNCaP (ATCC, Rockville, MD), were maintained and propagated in T25 flasks in RPMI 1640 media (GIBCO-BRL, Gaithersburg, MD) supplemented with 10% FCS (Gemini Bio-Products, Calabasas, CA), 2 mM glutamine, Fungizone (1 μg/ml) and penicillin (200 unit/ml)/ streptomycin (200 μg/ml) (referred as complete medium) in a 37°C humidified 5% CO 2 incubator.

  • Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.

  • Cells were grown and maintained in Dulbecco's Modified Eagle Medium (Life Technologies) that was supplemented with 10% fetal bovine serum (Hyclone), 170 mM glutamine and penicillin/streptomycin (100 μg/ml, Life Technologies) in humidified air containing 5% carbon dioxide at 37°C.

  • NIH 3T3 fibroblasts were grown in DME medium containing 1000 mg/liter glucose, supplemented with 10% FCS, 4 mM L-glutamine, penicillin (100 IU/ml), and streptomycin (100 μg/ml), at 37°C in a humidified atmosphere of 5% CO 2 in air.


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