Words similar to humidified
Example sentences for: humidified
How can you use “humidified” in a sentence? Here are some example sentences to help you improve your vocabulary:
Selection pressure was maintained by the presence of 1 mg/ml G418 sulfate (Life Technologies, Inc.) in the culture media, which was kept at 37°C in 7% CO 2 /humidified air and was changed every 3-4 days.
Cells were maintained in humidified air containing 5% CO 2 for two weeks to allow for cell-cell junctions to mature.
The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.
Human prostate carcinoma cells, LNCaP (ATCC, Rockville, MD), were maintained and propagated in T25 flasks in RPMI 1640 media (GIBCO-BRL, Gaithersburg, MD) supplemented with 10% FCS (Gemini Bio-Products, Calabasas, CA), 2 mM glutamine, Fungizone (1 μg/ml) and penicillin (200 unit/ml)/ streptomycin (200 μg/ml) (referred as complete medium) in a 37°C humidified 5% CO 2 incubator.
NIH 3T3 fibroblasts were grown in DME medium containing 1000 mg/liter glucose, supplemented with 10% FCS, 4 mM L-glutamine, penicillin (100 IU/ml), and streptomycin (100 μg/ml), at 37°C in a humidified atmosphere of 5% CO 2 in air.
Loading...