Words similar to horseradish
Example sentences for: horseradish
How can you use “horseradish” in a sentence? Here are some example sentences to help you improve your vocabulary:
After extensive washes with phosphate buffered saline, the blots were incubated with appropriate secondary antibodies (1:1000) conjugated with horseradish peroxidase for 1 hour at room temperature.
Previous studies have demonstrated that these antibodies recognize the phosphorylated (active) forms of extracellular-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK) Membranes were then washed (3 × 5 min each) with TBST buffer and incubated with anti-rabbit IgG horseradish peroxidase conjugate or anti-mouse IgG horseradish peroxidase conjugate (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at room temperature.
Membranes were then incubated with a secondary antibody, horseradish peroxidase-conjugated anti-rabbit IgG (1:4000, ICN Biomedical Research Products, Costa Mesa, CA, USA).
An anti-phosphotyrosine antibody conjugated to horseradish peroxidase (SC7020, Santa Cruz Biotechnology) was used for the detection of phosphotyrosine-containing proteins by immunoblotting.
Plates were left at 22°C under mild humidity for 2 hours on a shaker and then washed four times with PBS and blotted dry before addition of the secondary horseradish peroxidase-conjugated antibody (Sigma) in 3% nonfat dried milk in PBS.