Words similar to horseradish
Example sentences for: horseradish
How can you use “horseradish” in a sentence? Here are some example sentences to help you improve your vocabulary:
Monoclonal antibodies against PTEN were generated and used as described previously [ 44 ] . The secondary antibodies were coupled to horseradish peroxidase, and were detected by chemiluminescence using the SuperSignal™ substrate from Pierce (Rockford, MD).
Fibrosarcoma PAI-1 and wtPAI-1 were transferred onto nitrocellulose paper and the proteins were revealed with anti-human PAI-1 monoclonal antibodies, followed by horseradish peroxidase (HRP) conjugated rabbit anti-mouse antibody, and enhanced chemiluminescence (ECL).
Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.
Location of the primary antibodies was achieved by subsequent application of a biotinylated anti-primary antibody, an avidin-biotin complex conjugated to horseradish peroxidase, and diaminobenzidine (Vectastain Elite Kit, Vector, Burlingame, CA).
CAP, cellulose acetate phthalate; STD, sexually transmitted disease; ELISA, enzyme-linked immunosorbent assay; mAbs, monoclonal antibodies; FBS, fetal bovine serum; PEG 6000, polyethylene glycol 6000; HIV-1, human immunodeficiency virus type 1; BSA, bovine serum albumin; PBS, phosphate buffered saline; Ch-PBS, chicken serum (10%) in PBS; PBS-BG, 1% BSA/1% gelatin in PBS; HRP, horseradish peroxidase; sCD4, soluble CD4; TS, 0.14 M NaCl, 0.01 M Tris, pH 7.0; CTAP, cellotetraose acetate phthalate; pdb, Protein Data Bank; ED 50 , effective dose for 50 % inhibition.
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