Words similar to hooks
Example sentences for: hooks
How can you use “hooks” in a sentence? Here are some example sentences to help you improve your vocabulary:
Based on those results, we concluded that targeting sequences (hooks) with a combined length of up to 130 bp cloned into the ADH1 promoter region would allow selection for insertion events during TAR cloning.
To study in greater detail how the presence of nonhomologous DNA at the end of one of the hooks reduces gene capture efficiency, we constructed a set of TAR vectors with different lengths of nonhomologous DNA next to the hook.
The relatively large sizes of the inserts suggest that genomic alphoid DNA fragments recombine with targeting hooks in the vector by sequences located at DNA fragment ends, rather than by internal sequences.
A low yield is predicted for a TAR vector containing two unique targeting hooks.
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
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