Example sentences for: hoechst

How can you use “hoechst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Before and after activation, fused embryos were stained with 5 μg/ml Hoechst 33342 for 20 min and then examined for nuclear swelling and polar body formation under ultraviolet light.

  • Tissue sectionswere then counter-stained with Hoechst 33258 stain (1 μg/ml) (Sigma),and viewed with an epifluorescence microscope (Zeiss Axiophot) equippedwith filter sets for fluorescein and Hoechst staining.

  • Cells grown on coverslips were treated with H 2 O 2 for various periods of time as indicated in figure legends, fixed for 10 minutes with 3.7% formaldehyde (prepared in PBS), stained with 1 μg/ml bisbenzimide solution (Hoechst 33258, Sigma, St. Louis, MO) for 10 minutes at room temperature, mounted with GEL/MOUNT, then observed under a fluorescent microscope as described in the previous section.

  • These domains coincide with regions that stain with the blue fluorescent DNA-binding dye Hoechst 33342 (Fig.

  • Cells were stained 10-20 minutes with 5 μg/ml of the cell-permeable DNA binding dye Hoechst 33342.


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