Example sentences for: hoechst

How can you use “hoechst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Using Hoechst 33342, we assayed for potential toxicity of CDDO in human primary chondrocytes: we found decreased cell numbers and increased apoptosis at or above 5 μM CDDO (data not shown).

  • Cells were stained 10-20 minutes with 5 μg/ml of the cell-permeable DNA binding dye Hoechst 33342.

  • Eggs were then washed in M199 media and incubated in 10 μg/ml Hoechst chromatin stain at 37°C for 10 min.

  • Hoechst 33342 stains AT-rich DNA that concentrates around the centromeres [ 45 49 ] . Given the role of the centromere as a checkpoint for regulation of the mitotic phase of the cell cycle [ 50 51 ] , it was hypothesized that C/EBPα localization around the centromere could play a role in C/EBPα regulation of the cell cycle [ 49 ] .

  • The green fluorescence emitted from C/EBPα tagged with GFP and the blue fluorescence emitted from Hoechst 33342-stained DNA were readily distinguished by selectively exciting each fluorophore and capturing fluorescence emissions specific for each fluorophore using appropriate excitation and emission filter sets (Chroma Technology Corporation, Brattelboro, VT) [ 45 ] . The images shown in Fig.


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