Example sentences for: hoechst

How can you use “hoechst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Eggs were then washed in M199 media and incubated in 10 μg/ml Hoechst chromatin stain at 37°C for 10 min.

  • Cells stained with Hoechst 33258 indicated that 15d-PGJ 2 prevented H 2 O 2 induced nuclear condensation (Fig.

  • Cells grown on coverslips were treated with H 2 O 2 for various periods of time as indicated in figure legends, fixed for 10 minutes with 3.7% formaldehyde (prepared in PBS), stained with 1 μg/ml bisbenzimide solution (Hoechst 33258, Sigma, St. Louis, MO) for 10 minutes at room temperature, mounted with GEL/MOUNT, then observed under a fluorescent microscope as described in the previous section.

  • Hoechst chromatin stain was used at 10 μg/ml in PBS in order to determine which, if any, eggs had been activated or were undergoing degeneration as a consequence of manipulation.

  • The oocytes had been previously stained in the manipulation medium supplemented with 5 μg/ml Hoechst 33342 (Sigma) for 20 min and confirmation of successful enucleation was achieved by visualizing the cytoplast and removed cytoplasm under ultraviolet light.


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