Words similar to hoechst

Example sentences for: hoechst

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Cells grown on coverslips were treated with H 2 O 2 for various periods of time as indicated in figure legends, fixed for 10 minutes with 3.7% formaldehyde (prepared in PBS), stained with 1 μg/ml bisbenzimide solution (Hoechst 33258, Sigma, St. Louis, MO) for 10 minutes at room temperature, mounted with GEL/MOUNT, then observed under a fluorescent microscope as described in the previous section.
Cells were then simultaneously fixed with the addition of 830 μl of 0.5% paraformaldehyde in PBS and stained with the addition of 220 μl of 10 μg/ml Hoechst 33342 (Molecular Probes, Inc.) containing 5% Tween-20 (Sigma-Aldrich, Inc) for at least 16 hours at 4°C in the dark.
Tissue sectionswere then counter-stained with Hoechst 33258 stain (1 μg/ml) (Sigma),and viewed with an epifluorescence microscope (Zeiss Axiophot) equippedwith filter sets for fluorescein and Hoechst staining.
Hoechst chromatin stain was used at 10 μg/ml in PBS in order to determine which, if any, eggs had been activated or were undergoing degeneration as a consequence of manipulation.
Hoechst chromatin stain was utilized to determine whether eggs had parthenogenetically activated (activated eggs are mitotic and exhibit two chromatin poles rather than one, as indicated by an asterisk in Figs 3, 4, 5).