Words similar to hoechst
Example sentences for: hoechst
How can you use “hoechst” in a sentence? Here are some example sentences to help you improve your vocabulary:
Using Hoechst 33342, we assayed for potential toxicity of CDDO in human primary chondrocytes: we found decreased cell numbers and increased apoptosis at or above 5 μM CDDO (data not shown).
Cells were stained 10-20 minutes with 5 μg/ml of the cell-permeable DNA binding dye Hoechst 33342.
Eggs were then washed in M199 media and incubated in 10 μg/ml Hoechst chromatin stain at 37°C for 10 min.
Hoechst 33342 stains AT-rich DNA that concentrates around the centromeres [ 45 49 ] . Given the role of the centromere as a checkpoint for regulation of the mitotic phase of the cell cycle [ 50 51 ] , it was hypothesized that C/EBPα localization around the centromere could play a role in C/EBPα regulation of the cell cycle [ 49 ] .
The green fluorescence emitted from C/EBPα tagged with GFP and the blue fluorescence emitted from Hoechst 33342-stained DNA were readily distinguished by selectively exciting each fluorophore and capturing fluorescence emissions specific for each fluorophore using appropriate excitation and emission filter sets (Chroma Technology Corporation, Brattelboro, VT) [ 45 ] . The images shown in Fig.