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Example sentences for: hirsutum
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The catalytic subunit exists as two distinct genes with highly conserved exons and intron boundaries in twelve species of plants [ 25 ] . In the flowering plant Daucus carota (carrot) evidence exists for two distinct isoforms for the catalytic subunit, one of which is tonoplast specific while the other is localized to the Golgi [ 26 ] . In addition, two distinct mRNAs have been isolated from cotton ( Gossypium hirsutum ) [ 27 ] . Two subunit A mRNAs are expressed in the unicellular alga Acetabularia acetabulum [ 28 29 ] . Isoforms of other V-ATPase subunits are also known from plants including subunit B [ 27 30 32 ] , the proteolipid subunit [ 33 34 35 36 37 ] and subunits D and E [ 38 ] .
Of the species examined to date three have only a single subunit A gene detected, the alga Coleochaete scutata, sugar beet ( Beta vulgarise [ 63 64 ] , and A. thaliana [ 25 ] . Flowering plants shown to have at least two subunit A genes include oat ( Avena sativa ), carrot ( Daucus carota ), tobacco ( Nicotiana tabacum ), tomato ( Lycopersicon esculentum ), cotton ( Gossypium hirsutum ), magnolia ( Magnolia virginana ), Hydrastis canadensis, Chenopodium rubrum, and clematis ( Clematis liguisticfolio ). In addition, the unicellular alga Acetabularia acetabulum has two V-ATPase subunit A genes.
[ 16 ] observed this effect within a few hours after cotton ( Gossypium hirsutum L.) plants were transferred from 30 to 18°C.
Since etiolation results in rapid elongation of seedlings it was expected that there would be a concomitant increase in subunit A messenger RNA as seen with proteolipid subunit mRNA in A. thaliana [ 36 ] and subunit A message levels in rapidly expanding cotton ( Gossypium hirsutum ) fibers [ 73 ] . Equivalent loading of whole RNA was evaluated via spectrophotometry measuring absorption at 260 nm using a Perkin-Elmer Lambda-3 spectrophotometer and by visual confirmation using non denaturing ethidium bromide stained agarose gel electrophoresis (results not shown).
In order for being counted as exhibiting disagreement between the orthology bootstrap values and sequence similarity a query sequence had to fulfill the following two conditions: (i) it must have a least one ortholog with bootstrap orthology value above or equal to O , and (ii) all sequences in the alignment with bootstrap orthology values above N , must have distance ratios smaller or equal to R for at least one sequence with bootstrap orthology lower or equal to N . Sequences from the following species were ignored in this analysis (since they were the species of the query sequence or related to it): A. thaliana proteome: Rosidae ( A. thaliana, Pisum sativum , Glycine max , Cucurbita maxima , Cucumis sativus , Brassica campestris , Brassica napus , Citrus unshiu , Citrus sinensis , Theobroma cacao , Gossypium hirsutum ); C. elegans proteome: nematodes ( C. elegans , Caenorhabditis briggsae , Haemonchus contortus , Ascaris suum ).