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Example sentences for: hirsutum
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The catalytic subunit exists as two distinct genes with highly conserved exons and intron boundaries in twelve species of plants [ 25 ] . In the flowering plant Daucus carota (carrot) evidence exists for two distinct isoforms for the catalytic subunit, one of which is tonoplast specific while the other is localized to the Golgi [ 26 ] . In addition, two distinct mRNAs have been isolated from cotton ( Gossypium hirsutum ) [ 27 ] . Two subunit A mRNAs are expressed in the unicellular alga Acetabularia acetabulum [ 28 29 ] . Isoforms of other V-ATPase subunits are also known from plants including subunit B [ 27 30 32 ] , the proteolipid subunit [ 33 34 35 36 37 ] and subunits D and E [ 38 ] .
In order for being counted as exhibiting disagreement between the orthology bootstrap values and sequence similarity a query sequence had to fulfill the following two conditions: (i) it must have a least one ortholog with bootstrap orthology value above or equal to O , and (ii) all sequences in the alignment with bootstrap orthology values above N , must have distance ratios smaller or equal to R for at least one sequence with bootstrap orthology lower or equal to N . Sequences from the following species were ignored in this analysis (since they were the species of the query sequence or related to it): A. thaliana proteome: Rosidae ( A. thaliana, Pisum sativum , Glycine max , Cucurbita maxima , Cucumis sativus , Brassica campestris , Brassica napus , Citrus unshiu , Citrus sinensis , Theobroma cacao , Gossypium hirsutum ); C. elegans proteome: nematodes ( C. elegans , Caenorhabditis briggsae , Haemonchus contortus , Ascaris suum ).
Since etiolation results in rapid elongation of seedlings it was expected that there would be a concomitant increase in subunit A messenger RNA as seen with proteolipid subunit mRNA in A. thaliana [ 36 ] and subunit A message levels in rapidly expanding cotton ( Gossypium hirsutum ) fibers [ 73 ] . Equivalent loading of whole RNA was evaluated via spectrophotometry measuring absorption at 260 nm using a Perkin-Elmer Lambda-3 spectrophotometer and by visual confirmation using non denaturing ethidium bromide stained agarose gel electrophoresis (results not shown).
[ 16 ] observed this effect within a few hours after cotton ( Gossypium hirsutum L.) plants were transferred from 30 to 18°C.
Of the species examined to date three have only a single subunit A gene detected, the alga Coleochaete scutata, sugar beet ( Beta vulgarise [ 63 64 ] , and A. thaliana [ 25 ] . Flowering plants shown to have at least two subunit A genes include oat ( Avena sativa ), carrot ( Daucus carota ), tobacco ( Nicotiana tabacum ), tomato ( Lycopersicon esculentum ), cotton ( Gossypium hirsutum ), magnolia ( Magnolia virginana ), Hydrastis canadensis, Chenopodium rubrum, and clematis ( Clematis liguisticfolio ). In addition, the unicellular alga Acetabularia acetabulum has two V-ATPase subunit A genes.
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