Words similar to high-stringency

Example sentences for: high-stringency

How can you use “high-stringency” in a sentence? Here are some example sentences to help you improve your vocabulary:

We used a high-stringency touchdown protocol in which the annealing temperature was lowered progressively from 60°C to 50°C in 2°C steps over the first six cycles [ 52].
Membranes were washed twice at 65°C for 30 min in high-stringency buffer containing 40 mM Na 2 HPO 4 (pH 7.2), 1 mM EDTA (pH 8.0) and 1% SDS, before exposure to autoradiographic film.
Highest sensitivity and cleanest background was obtained with overnight 37°C incubations followed by high-stringency washes (see below).
The issue of cross-hybridisation of related genes on cDNA microarrays was addressed by Miller [ 9 ] . These authors used high-stringency conditions and found that minimal cross-hybridisation occurred among genes with up to 94% sequence identity.