Words similar to hesmpcs
Example sentences for: hesmpcs
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Therefore, we tested whether hESMPCs have the same potential (Figure 2).
We did not detect markers of undifferentiated hESCs, such as Nanog [15] or Oct-4 [16], in any of the hESMPCs by RT-PCR (see Figure 2D) and immunocytochemistry (data not shown), suggesting the lack of any undifferentiated ES cells in hESMPC cultures.
Adipocytic differentiation of hESMPCs was induced under conditions described previously for primary adult MSCs [5].
These data demonstrate that hESMPCs can give rise to mesenchymal derivatives typically obtained from primary adult MSCs, as well as to cells expressing markers of skeletal muscle.
hESMPCs were plated at low density (1 × 10 3 to 2.5 × 10 3 cells/cm 2 ) on tissue-culture-treated dishes in the presence of 10 mM β-glycerol phosphate (Sigma), 0.1 μM dexamethasone, and 200 μM ascorbic acid in alpha MEM medium containing 10% FBS for 3–4 wk.