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Example sentences for: herrington

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Herrington and colleagues have specifically examined T m as it applies to ISH and have defined the term "tissue T m ", ( T m t), having noted that the theoretical T m (derived from analysis of solution kinetics) did not match the empirically derived value for T m in HPV ISH assays using full-length probes, suggesting that hybridization involving tissue sections involves additional determinants of T m . After several studies [ 6 7 8 ] it was concluded that the main difference between T m and T m tis (probably) due to the use of a different end-point for measurement (e.g.
a chromogenic stain following application of intermediary reagents) rather than biochemical alteration of DNA-DNA interactions (measured by spectrophotometry) [ 8 ] . It was also concluded that mismatched probe targets should be removed using empirically derived post-hybridization washing conditions in order to achieve specificity and that appropriate conditions should be determined by experiment rather than calculated theoretically from T m . In a later study Herrington et al.