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Females lack an estrous cycle and anestrous adults are dependent upon direct contact with a non-volatile male pheromone for induction of estrus [ 8 ] . Hormonal control of scentmarking and sexual behavior of this species (hereinafter, opossum) has been described in some detail [ 9 10 ] . Estrus is induced when females nuzzle scentmarks from the androgen-dependent suprasternal (chest) gland [ 10 11 ] . Nuzzling delivers nonvolatile chemical signals to the vomeronasal organ (VNO) [ 12 ] and surgical removal of the VNO prevents induction of estrus by scentmarks alone [ 13 ] , although cautery ablation of the VNO fails to prevent ovarian activation and ovulation in females that are caged with adult males [ 14 ] . While anestrous adults are reliant on male pheromone for reproductive activation, spontaneous postlactational estrus occurs in the absence of direct contact with male pheromone [ 15 ] .
27, 1999, at 29-43 (comment of Bill Beardall, Texas Rural Legal Aid) [hereinafter March Comments]; April Comments at 53-58 (comment of Howard Berman, Member of Congress); see also discussion infra Part III(B)(2).
In addition to the similar conservation pattern, separate secondary structure predictions for both the eukaryotic RAD52 family and their potential bacterial homologs showed a complete concordance of the predicted structural elements between RAD52 and the bacterial proteins, strongly suggesting that they all belong to a single homologous superfamily (hereinafter the RAD52 superfamily).
The most straightforward version of this type of analysis, to which we hereinafter refer to as 'genome-tree' building, involves scaling-up the traditional tree-building approach and analyzing the phylogenetic trees for multiple gene families (in principle, all families represented in many genomes), in an attempt to derive a consensus, 'organismal' phylogeny [ 3 4 5 ] . However, because of the wide spread of horizontal gene transfer and lineage-specific gene loss, at least in the prokaryotic world, comparison of trees for different families and consensus derivation may become highly problematic [ 6 7 ] . Probably due to all these problems, a pessimistic conclusion has been reached that prokaryotic phylogeny might not be reconstructable from protein sequences, at least with current phylogenetic methods [ 4 ] .
Department of Labor, Division of Foreign Labor Certifications, Revised June 1999 [hereinafter FY 1998 H-2A Report].
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