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Example sentences for: hercules

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Membrane protein concentrations were determined by a colorimetric assay according to manufacturer's protocol (BioRad, Hercules, CA) and 20 μg of total membrane proteins resolved by 7.5% SDS acrylamide gel.
Antisera were screened for their ability to detect the immunizing peptide and were immunoaffinity-purified with columns of the recombinant fragment covalently bound to a Affi-Gel 15 matrix (Bio-Rad, Hercules, CA).
Equal quantities of protein, as determined by a protein assay (BioRad, Hercules, CA), were separated by electrophoresis in a 12% SDS-polyacrylamide gel.
10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.
Consistency of the HIV-EIA results was maintained by confirmatory testing according to a protocol that was in use at the study center - one of 10 HIV-1 EIA positive and one of 25 HIV-1 negative sera underwent confirmatory testing by HIV-1 Western immunoblotting (BioRad Novapath, Hercules, California, USA).