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Example sentences for: hela
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These results were also reproduced in HeLa S3 and mouse J774A.
HeLa cells were grown in DMEM (Gibco/BRL) supplemented with 10% fetal calf serum, seeded at 10 cells/well in 96-well microtiter plates and transfected 8 hours later using Exgen 500 (Euromedex, Souffelweyersheim, France).
Cell proliferation was measured by [ 3H]-thymidine uptake [ 8 ] . HeLa S3 cells (3 × 10 4cells/well) were incubated in 96-well plates in the presence of varying concentrations of the tetrapyrroles for 2 days at 37°C, after which 3H-TdR (1 μCi/well) was added.
demonstrated that 5-25 μM PD98059 could prevent H 2 O 2 induced cell death in an oligodendrocyte cell line [ 14 ] . In contrast, 20 μM PD98059 was shown to enhance cell death caused by H 2 O 2 treatment in HeLa cells [ 15 ] . This reagent had no significant effect on H 2 O 2 treated ARPE-19 cells under our experimental conditions.
To determine the localization of BTBD2 in cells, we transiently transfected HeLa cells with expression plasmids encoding either myc-tagged full length BTBD2 (m-BTBD2F) or a truncated BTBD2 having the N-terminal half of the BTB domain removed (m-BTBD2T).
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