Words similar to hebd

Example sentences for: hebd

How can you use “hebd” in a sentence? Here are some example sentences to help you improve your vocabulary:

To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.
The beads were incubated with 250 ng of GST-T7-Cdc14 in 100 μl of HEBD for 1 hr, followed by the same sequence of washes.
The beads were washed once with 70 μl of HEBD, and the wash was pooled with the supernatant, and precipitated with TCA (10% final) on ice for 30 min.
To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.