Words similar to heat-inactivated
Example sentences for: heat-inactivated
How can you use “heat-inactivated” in a sentence? Here are some example sentences to help you improve your vocabulary:
HeLa and A549 cells were grown in Dulbecco's modified Eagle's medium containing 1 g of glucose/liter (DMEM) and 10 % heat-inactivated FBS.
The FLS cultures were established from synovial tissues obtained during joint replacement surgery in RA patients [ 6 ] . The FLS were cultured in DMEM plus 10% heat-inactivated human AB serum (BioWhittaker, Walkersville, MD, USA), 10% fetal bovine serum, penicillin, streptomycin, and L-glutamine.
Murine fibroblast NIH 3T3 cells, amphotropic PA317 packaging cells, and Phoenix E ecotropic packaging cells were cultured in Dulbecco's modified Eagle's medium (DMEM)-high glucose (GIBCO-BRL, Grand island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO-BRL, Grand island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, and 200 mM L-glutamine.
The syk -negative DT40 chicken B cell line was obtained from T. Kurosaki and was grown in RPMI supplemented with 2 mM L-glutamine, 55 μM β-mercaptoethanol, 10 % heat-inactivated fetal calf serum, and 2 % heat-inactivated chicken serum.
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
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